Molecular cloning and expression analysis of a monosaccharide transporter gene OsMST4 from rice (Oryza sativa L.)
| Autor: | Wang, Y., Xu, H., Wei, X., Chai, C., Xiao, Y., Zhang, Y., Chen, B., Xiao, G., Ouwerkerk, P.B.F., Wang, M., Zhu, Z. |
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| Přispěvatelé: | TNO Kwaliteit van Leven |
| Jazyk: | angličtina |
| Rok vydání: | 2007 |
| Předmět: |
Plant cell culture
molecular cloning Biomedical Research DNA Complementary Monosaccharide Transport Proteins Physiology Molecular biology Molecular Sequence Data complementary DNA Crops Oryza sativa Fructose Saccharomyces cerevisiae chemistry phylogeny reverse transcription polymerase chain reaction gene expression profiling Oryza sativa L genetics Seed development vegetable protein Amino Acid Sequence RNA Messenger primer DNA Cloning Molecular Biology In Situ Hybridization DNA Primers Plant Proteins Monosaccharide transporter Base Sequence Sequence Homology Amino Acid messenger RNA Reverse Transcriptase Polymerase Chain Reaction rice article food and beverages nucleotide sequence sequence homology glucose transporter Genes molecular genetics cDNA cloning Gene expression Transcription Cloning |
| Zdroj: | Plant Molecular Biology, 4, 65, 439-451 |
| Popis: | Monosaccharide transporters mediate the membrane transport of a variable range of monosaccharides, which plays a crucial role in sugar distribution throughout the plant. To investigate the significance of monosaccharide transporters for rice (Oryza sativa L.) seed development, cDNA of a new putative monosaccharide transporter gene OsMST4 was isolated. The deduced OsMST4 protein shows typical features of monosaccharide transporters, and shares high homology with other plant homologues. Heterologous expression in yeast (Saccharomyces cerevisiae) showed that OsMST4 is a functional monosaccharide transporter capable of transporting glucose, fructose, mannose and galactose. Transcriptional analysis revealed that OsMST4 is expressed in all tested organs/tissues. In developing caryopses, its expression is high at the early and middle grain filling stages, and declines gradually to low levels after that. Further analysis revealed that it is expressed in both the maternal tissue and the filial tissue, with its highest expression in embryo. Cellular location in young caryopses through RNA in situ hybridization showed that OsMST4 mRNA mainly accumulates in the vascular parenchyma of the chalazal vein, cross-cells, nucellar tissue and endosperm. The expression pattern of OsMST4 was further confirmed by histochemical analysis of the OsMST4-promoter-β-glucuronidase (GUS) transgenic rice plants. These data indicate that OsMST4 is actively involved in monosaccharides supply for seed development during the course of grain filling. In addition, the cell type-specific expression patterns of OsMST4 in other sink and source tissues were also investigated, and its corresponding physiological roles were discussed. © 2007 Springer Science+Business Media B.V. |
| Databáze: | OpenAIRE |
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