Variation in the composition of expoloysaccharides produced by Lactiplantibacillus plantarum strain Lbio28 based on the fermentation medium

Autor: Bachtarzi, Nadia, Immacolata, Speciale, Kharroub, Karima, De Castro, Cristina, Ruíz García, Lorena, Ruas-Madiedo, Patricia
Rok vydání: 2021
Předmět:
Zdroj: Digital.CSIC. Repositorio Institucional del CSIC
instname
Popis: Resumen del trabajo presentado en el 1st International Conference on Applied Engineering and Natural Sciences, celebrado en Konya (Turquía), del 1 al 3 de noviembre de 2021
Some strains of lactic acid bacteria excrete carbohydrate polymers during their growth, which can either remain attached to the cell membrane, the capsular polysaccharides (CPS), or be released into the fermentation medium, the exopolysaccharides (EPS). These biopolymers offer a natural alternative to commercial food additives due to their physico-chemical characteristics. Indeed, their in situ production in food matrices has many advantages, as they improve rheological properties, such as increasing viscosity, reducing syneresis and improving texture, in addition to having emulsifying, thickening and stabilising properties. In this work, a strain (Lbio28) of lactic acid bacteria producing ropy EPS was isolated from fermented milk from the BordjBouArreridj area (Algeria) and identified as Lactiplantibacillus plantarum. The polymers produced by this strain on two fermentation media, MRS agar and milk medium, were extracted and purified by two methods. Their molecular weights and compositions were then defined by exclusion size chromatography (SEC-MALLS) and gas chromatography-mass spectroscopy (GC-MS), respectively. The results obtained showed that the polymers produced on these two media are heteropolymers with different molecular weights, 1.9×105 Da on MRS and 1.18×106 Da on milk medium. The polymer produced on MRS medium was composed of glucose, galactose and glycerol, whereas the second polymer produced on milk medium, in addition to the three monomers mentioned above, included ribitol, mannose, neuraminic acid, glucosamine and galactosamine. These results show that the fermentation medium used influences the composition of the exopolysaccharide produced and then its physico-chemical and techno-functional characteristics
Databáze: OpenAIRE
Externí odkaz: