Gene silencing strategies to increase HIV-1 VLP production in HEK 293 cells

Autor:	Rigau Granés, Cristina
Přispěvatelé:	Universitat Autònoma de Barcelona. Escola d'Enginyeria, Gòdia Casablancas, Francesc, Cervera Gracia, Laura
Jazyk:	angličtina
Rok vydání:	2019
Předmět:	viruses Transformacions genètiques virus diseases 575 - Genètica general. Citogenètica general. Immunogenètica. Evolució. Filogènia Genètica
Zdroj:	RECERCAT (Dipòsit de la Recerca de Catalunya) Recercat. Dipósit de la Recerca de Catalunya instname
Popis:	The HIV-1 Gag polyprotein, recombinantly expressed in mammalian cell platforms, is self-assembled, generating HIV-1 Gag virus like particles (VLPs). The expression of HIV-1 Gag polyprotein is achieved by DNA/PEI-based transient transfection of HEK 293 cells in suspension. In this study, the use of small interfering RNA (siRNA) has been investigated as an alternative to the use of VPA and caffeine as production enhancers in HIV-1 Gag VLP production. Two shRNA sequences against HDAC5 and PDE8A genes, cloned in the expression vector containing Gag-GFP gene, were tested.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=dedup_wf_001::ceb8b61c8e97c94ed689da9caa278655 https://hdl.handle.net/2072/351507 Zobrazit plný text záznamu