Combination of blood and biphasic calcium phosphate microparticles for the reconstruction of large bone defects in dog: A pilot study

Autor: Balaguer, Thierry, Fellah, Borhane H., Boukhechba, Florian, Traverson, Marine, Mouska, Xavier, Ambrosetti, Damien, Dadone, Berengere, Michiels, Jean-Francois, Amri, Ez-Zoubir, Trojani, Christophe, Bouler, Jean-Michel, Gauthier, Olivier, Rochet, Nathalie
Přispěvatelé: Génétique, physiopathologie et ingénierie du tissu osseux (GéPITOS), Centre National de la Recherche Scientifique (CNRS)-Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Université Côte d'Azur (UCA), Graftys SARL, Institut de pharmacologie moléculaire et cellulaire (IPMC), Hôpital Pasteur [Nice] (CHU), Université Nice Sophia Antipolis - Faculté de Médecine (UNS UFR Médecine), Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA), Institute of Developmental Biology and Cancer (IBDC), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS)-Université Côte d'Azur (UCA), Service de Chirurgie Orthopédique et Chirurgie du Sport, Hôpital de L'Archet, University of Nice-Sophia Antipolis, Chimie Et Interdisciplinarité : Synthèse, Analyse, Modélisation (CEISAM), Université de Nantes - UFR des Sciences et des Techniques (UN UFR ST), Université de Nantes (UN)-Université de Nantes (UN)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC), Dauphine Recherches en Management (DRM), Université Paris Dauphine-PSL, Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS), Génétique et pathologies moléculaires (GPM)
Jazyk: angličtina
Rok vydání: 2018
Předmět:
Zdroj: Journal of Biomedical Materials Research Part A
Journal of Biomedical Materials Research Part A, Wiley, 2018, 106 (7), pp.1842-1850. ⟨10.1002/jbm.a.36384⟩
ISSN: 1549-3296
1552-4965
DOI: 10.1002/jbm.a.36384⟩
Popis: We previously reported that biphasic calcium phosphate (BCP) microparticles embedded in a blood clot induces ectopic bone formation in mice and repairs a critical femoral defect in rat. The present pilot study aimed to evaluate in dog and in two models of large defects the efficacy of this composite named blood for reconstruction of bone (BRB). We show here that BRB is a cohesive biomaterial easy to prepare from dog autologous blood and to mold to fill large bone defects. First in a model of cylindrical femoral condyle defect, the BRB was compared with BCP particles alone. After 8 weeks, this revealed that the amount of mature bone was slightly and significantly higher with BRB than with BCP particles. Second, in a model consisting in a 2 cm-long critical interruptive defect of the ulna, the BRB was compared with autologous bone. After 6 months, we observed that implantation of BRB can induce the complete reconstruction of the defect and that newly formed bone exhibits high regenerative potential. Comparison with the results obtained with autologous bone grafting strongly suggests that the BRB might be an efficient biomaterial to repair large bone defects, as an alternative or in addition to autologous bone. (c) 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 1842-1850, 2018.
Databáze: OpenAIRE
Externí odkaz: