| Autor: |
Dorian Forte, DARIA SOLLAZZO, MARTINA BARONE, CRISTINA MORSIANI, Ricci, F., Carloni, Sara, Fabbri, F., Auteri, G., Romano, M., Ottaviani, M., Tazzari, P. L., MICHELE CAVO, GIOVANNI MARTINELLI, Claudio Franceschi, Vianelli, N., Miriam Capri, FRANCESCA PALANDRI, Lucia Catani |
| Přispěvatelé: |
D. Forte, D. Sollazzo, M. Barone, C. Morsiani, F. Ricci, S. Carloni, F. Fabbri, G. Auteri, M. Romano, M. Ottaviani, P.L. Tazzari, M. Cavo, G. Martinelli, C. Franceschi, N. Vianelli, M. Capri, F. Palandri, L. Catani |
| Jazyk: |
angličtina |
| Rok vydání: |
2018 |
| Předmět: |
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| Zdroj: |
Alma Mater Studiorum Università di Bologna-IRIS |
| Popis: |
Introduction: Myelofibrosis (MF) is a myeloproliferative neoplasm (MPN) characterized by clonal hemopoiesis, inflammatory microenvironment and mutations in JAK2, MPL, or CALR genes. Around 10% of patients (pts) do not carry the 3 mutations (Triple negative (TN)). Microparticles (MPs; 0.1-1 μm) are small vesicles deriving from plasma membrane during homeostasis and cell activation of a wide variety of cells with a role in intercellular signalling. Circulating MPs are increased in inflammation and cancer including MPN. However, their role in the pathogenesis of MF is still elusive. Here we compared phenotype, function and microRNAs (miRs) expression of isolated MPs of JAK2V617F mutated and TN pts. Methods: Peripheral blood was collected from 10 MF pts (at diagnosis or out of cytotoxic treatment for at least 3 months) and 10 age/sex-matched healthy donors (HD). MF pts were JAK2V617F mutated (n=5) and TN n=5). MPs were purified from 2 mL of platelet poor plasma by ultracentrifugation and quantified using Nanosight technology. Isolated MPs were characterized by flow cytometry (CytoFLEX, Beckman Coulter) for the expression of platelets (PLT:CD61+CD62P+) and megakaryocytes (MK:CD61+CD62P-) markers. MPs were then analyzed for their ability to modulate the in vitro viability of immunomagnetically isolated CD34+ cells from MF pts and cord blood (CB). MiRs expression of isolated MPs (10e9) from 3 JAK2V617F mutated, 3 TN pts and 3 HD was investigated using TaqMan™ Array Human MicroRNA A Cards (ThermoFisher). Results: The mean number of plasma MPs isolated from MF pts was similar to HD (5*10e10/mL). However, flow cytometry analysis revealed that the mean percentage of PLT-MPs was significantly increased (p |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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