New insights in the genome content of the bacterium Mycobacterium brumae
| Autor: | Renau-Mínguez, Chantal, Herrero-Abadía, Paula, Sentandreu, Vicente, Ruiz-Rodríguez, Paula, Goig, Galo A., Comas, Iñaki, Julián, Esther, Coscollá, Mireia |
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| Rok vydání: | 2021 |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname |
| Popis: | Resumen del póster presentado al 41st Annual Congress of the European Society of Mycobacteriology (ESM), celebrado de forma virtual del 28 al 29 de junio de 2019. Mycobacterium brumae is a fast-growing, non-pathogenic Mycobacterium species, originally isolated from environmental and human samples in Barcelona, Spain. Previous studies have shown that the implementation of non-pathogenic mycobacteria, such as Mycobacterium bovis BCG can improve the treatment against high-risk non-muscle invasive bladder cancer (BC) by intravesical administration. M. brumae has been shown to be non-pathogenic and its phenotype and immunogenic effect have been well characterized. However, the knowledge of its underlying genetic composition is still incomplete. In this study we have sequenced the genome by means of PACBIO of the M. brumae strain CR-270 obtaining the most complete assembly to date. We describe its genetic content by showing evolutionary relationships between different mycobacteria and we compare its virulence gene content with other virulent mycobacteria such as H37Rv reference strain. Furthermore, we describe the genetic variability of M. brumae by comparative genomics using obtained Illumina sequences. Our results contribute to increase the knowledge about the genetic bases that explain the non-pathogenic phenotype of this bacterium with therapeutic potential. |
| Databáze: | OpenAIRE |
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