Deciphering copper resistance in Xanthomonas citri pv. citri

Autor: Richard, Damien, Ravigné, Virginie, Rieux, Adrien, Facon, Benoit, Boyer, Karine, Grygiel, Pierre, Javegny, Stéphanie, Terville, Marie Annabelle, Canteros, B.I., Robène, Isabelle, Vernière, Christian, Chabirand, Aude, PRUVOST, Olivier, Lefeuvre, Pierre
Přispěvatelé: Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad), Peuplements végétaux et bioagresseurs en milieu tropical (UMR PVBMT), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut de Recherche pour le Développement (IRD)-Institut National de la Recherche Agronomique (INRA)-Université de La Réunion (UR), LSV, Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), Instituto Nacional de Tecnología Agropecuaria (INTA), Biologie et Génétique des Interactions Plante-Parasite (UMR BGPI), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), ProdInra, Migration, Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)
Jazyk: angličtina
Rok vydání: 2017
Předmět:
Zdroj: 15th Congress of the Mediterranean Phytopathological Union: Plant health sustaining Mediterranean ecosystems Abstracts book
15. Congress of the Mediterranean Phytopathological Union
15. Congress of the Mediterranean Phytopathological Union, Jun 2017, Cordoba, Spain
Popis: International audience; Copper-based compounds are widely used in integrated pest management (1PM) programs aiming at controlling agriculturally important plant bacterial pathogens and the latter have adapted in response to this selective pressure. Copper resistance of Xanthomonas citri pv. citri (Xcc), a major citrus pathogen worldwide causing Asiatic citrus canker, was first observed in Argentina two decades ago and subsequently reported as a coplAB-based, plasmid-encoded system. The emergence of resistant strains has since been reported in Réunion (South West lndian Ocean) and Martinique (Eastern Caribbean Sea). Disease severity was found markedly increased in groves established with susceptible cultivars and infected with copper-resistant Xcc. Using tandem repeat-based genotyping and coplAB PCR, we demonstrated that the genetic structure of the copper-resistant strains from these three regions was made up of two distant clusters and varied for the detection of coplAB amplicons. ln order to investigate this pattern more closely, we sequenced six copper-resistant Xcc strains from Argentina, Martinique and Réunion, together with reference copper-resistant Xanthomonas and Stenotrophomonas strains using long-read sequencing technology. Genes involved in copper resistance were found to be straindependent with the novel identification in Xcc of copABCD and a eus heavy metal efflux resistancenodulation- division system. The genes providing the adaptive trait were part of a mobile genetic element similar to Tn3-like transposons and included in a conjugative plasmid. The mining of all bacterial genomes available from public databases suggested that the mobile elements containing copper resistance genes and their plasmid environments were primarily detected in the Xanthomonadaceae family.
Databáze: OpenAIRE
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