Influence of lactation stages on haematological and biochemical parameters in blood of Lacaune dairy sheep

Autor: Antunović, Zvonko, Mioč, Boro, Klir Šalavardić, Željka, Širić, Ivan, Držaić, Valentino, Đidara, Mislav, Džaja, Ana, Novoselec, Josip
Jazyk: angličtina
Rok vydání: 2022
Předmět:
Zdroj: Mljekarstvo : časopis za unaprjeđenje proizvodnje i prerade mlijeka
Volume 72
Issue 4
ISSN: 0026-704X
1846-4025
Popis: The research objective was to determine haematological and biochemical parameters in blood of 30 Lacaune dairy sheep during different stages of lactation (early: 60th, medium: 120th and late: 180th days). The sheep were on average 4 years old, in 3rd lactation. Haematological parameters were determined in whole blood (white blood cell (WBC), red blood cell (RBC), haemoglobin (HGB) concentration, haematocrit (HCT) value, mean corpuscular volume (MCV), mean cell haemoglobin (MCH), mean cell haemoglobin concentration (MCHC) and platelet (PLT) count), and a blood smear was used for determination of differential blood cell count. Determined biochemical parameters in blood serum were: concentration of minerals (Ca, P-inorganic, Mg and Fe), concentration of total proteins (TP), albumin (ALB), globulins (GLOB), urea, glucose (GUK), cholesterol (CHOL), high-density lipoprotein (HDL), low-density lipoprotein (LDL), triglycerides (TGC), beta-hydroxybutyrate (BHB) and non-esterified fatty acids (NEFA), enzyme activities (aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), γ-glutamyl transferase (GGT), creatine kinase (CK), activity of glutathione peroxidase (GPx) and total superoxide dismutase (SOD). With progression of lactation stages, sheep blood tests proved significant increase in WBC, RBC, HGB, HCT, monocytes, Fe, urea, TP, ALB, CHOL, HDL, LDL, BHB, AST, GGT and ALT as well as a decrease in lymphocytes, Mg, GUK and SOD. Determined changes in haematological and biochemical blood parameters of Lacaune dairy sheep during different lactation stages proved good quality nutrition of sheep and indicate the need to include this dairy breed/genotype in prospective schemes for definition of referential values for these indicators in sheep.
Cilj je ovoga rada utvrditi hematološke i biokemijske pokazatelje u krvi 30 lakon ovaca tijekom različitih stadija laktacije (rani: 60., srednji: 120. i kasni: 180. dan). Ovce su bile prosječne dobi 4 godine u 3. laktaciji. U punoj krvi su utvrđeni hematološki pokazatelji (broj leukocita: (WBC), eritrocita (RBC), hemoglobin (HGB), hematokrit (HCT), prosječni volumen eritrocita (MCV), prosječni hemoglobin u eritrocitu (MCH), prosječna koncentracija hemoglobina u eritrocitu (MCHC) i trombociti (PLT), a iz krvnih razmaza i diferencijalna krvna slika. Od biokemijskih pokazatelja u serumu utvrđeni su: koncentracije minerala (Ca, P-anorganski, Mg i Fe), ukupnih protein (TP), albumin (ALB), globulina (GLOB), uree, glukoze (GUK), kolesterola (CHOL), HDL-kolesterola (HDL), LDL-kolesterola (LDL), triglicerida (TGC), beta-hidroksibutirata (BHB) i neesterificiranih masnih kiseline (NEFA) te aktivnost enzima (aspartat aminotransferaze (AST), alanin aminotransferazee (ALT), alkalne fosfataze (ALP), γ-glutamil transferaze (GGT), kreatin kinaze (CK), glutation peroksidaze (GPx) i superoksid dismutaze (SOD). Utvrđeno je značajno povećanje WBC, RBC, HGB, HCT, Fe, urea, TP, ALB, CHOL, HDL, LDL, BHB, AST, GGT i ALT kao i smanjenje limfocita, Mg, GUK i SOD u krvi kako je laktacija odmicala. Utvrđene promjene u hematološkim i biokemijskim pokazateljima u krvi lakon ovaca tijekom laktacije ukazuju na kvalitetan obrok kojim su hranjene ovce, ali i potrebu uključivanja pasmine/genotipa osobito mliječnoga predznaka u buduće sheme izrade referentnih vrijednosti za navedene pokazatelje u ovaca.
Databáze: OpenAIRE
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