Autor: |
Vliegenthart, J.F.G., Bouwstra, J.B., Spoelstra, E.C., Waard, P. de, Leeflang, B.R., Kamerling, J.P. |
Jazyk: |
angličtina |
Rok vydání: |
1990 |
Předmět: |
|
Zdroj: |
European journal of biochemistry, 190(1), 113. Blackwell Synergy |
ISSN: |
0014-2956 |
Popis: |
1H- and 13C-NMR assignments for the carbohydrate part of the glycopeptide alpha-d-Man-(1->6)-[ß-d-Xyl-(1->2)]-ß-d-Man-(1->4)-ß-d-GlcNAc-(1->4)-[alpha-l-Fuc-(1->3)]-ß-d- GlcNAc-(1->N)-Asn~, derived from the proteolytic enzyme bromelain (EC 3.4.22.4), have been obtained using homo- and heteronuclear correlation spectroscopy, two-dimensional homonuclear Hartmann-Hahn and nuclear Overhauser enhancement experiments. A conformational model for the carbohydrate chain, deduced from the NMR data and consistent with hard-sphere exo-anomeric calculations shows that the rotamer population about the C-5C-6 bond of ß-Man is restricted to the omega=180 rotamer, mainly. |
Databáze: |
OpenAIRE |
Externí odkaz: |
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