| Autor: |
CASTALDO, CLOTILDE, NURZYNSKA, DARIA ANNA, DI MEGLIO, FRANCA, MONTAGNANI, STEFANIA, Vitale S, Cesselli D, Beltrami AP, Muller P |
| Přispěvatelé: |
Castaldo, Clotilde, Nurzynska, DARIA ANNA, DI MEGLIO, Franca, Vitale, S, Cesselli, D, Beltrami, Ap, Muller, P, Montagnani, Stefania |
| Jazyk: |
angličtina |
| Rok vydání: |
2005 |
| Popis: |
On the basis of the previous studies which described in the myocardium the presence of primitive cells that in vitro differentiate in the myocardial lineages, we identified by immunofluorescence in the adult hearts of healthy donors (n=10) and diseased hearts (ischemic and dilated cardiomyopathy, n=7) the population of c-kit (+) cells whose localization and phenotype differed between the groups. In the normal hearts c-kit(+) cells were observed mainly in the epicardial layer of ventricles. These cells were expressing transcription factors GATA-4 and MEF2C and were negative for alpha-sarcomeric actin. Confocal microscopy revealed the co-localization of alpha laminin and alpha4 integrin chain on these cells, but the western blot analysis showed the differences in the laminin expression between normal and pathological hearts: in the latter the level of expression was significantly higher and interestingly isoform 1 of laminin (characteristic for fetal, developing heart) was present. In the pathological hearts the c-kit(+) cells were not longer found in the epicardial region, but their presence was evidenced in the main myocardium. These cells also expressed MEF2C and GATA-4, but, in contrast with those found in the epicardial region of normal hearts, were alphasarcomeric actin positive. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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