Inhibition of phosphatidylcholine synthesis precedes apoptosis induced by C-2-ceramide: protection by exogenous phosphatidylcholine
| Autor: | Belén Ramos, Salido, G. M., Campo, M. L., Enrique Claro |
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| Rok vydání: | 2000 |
| Předmět: | |
| Zdroj: | Neuroreport r-FSJD. Repositorio Institucional de Producción Científica de la Fundació Sant Joan de Déu instname Universitat Autònoma de Barcelona r-FSJD: Repositorio Institucional de Producción Científica de la Fundació Sant Joan de Déu Fundació Sant Joan de Déu |
| ISSN: | 0959-4965 |
| Popis: | Cerebellar granule neurons in primary culture underwent apoptosis when exposed to C-2-ceramide. Addition of exogenous phosphatidylcholine (PtdCho) resulted in a dose-dependent full prevention of neuronal death. Exogenous PtdCho also prevented apoptosis induced by farnesol, N-oleoylethanolamine, and sphingomyelinase, but did not prevent apoptosis induced after lowering the potassium concentration in the medium to non-depolarizing levels. Moreover, C-2-ceramide inhibited labeling of [P-32]PtdCho in cells incubated with [P-32]orthophosphate, with the same potency to that causing apoptosis. Although cell viability did not decrease during the first few hours, inhibition of PtdCho synthesis was already patent after a 1 h exposure to C-2-ceramide. Taken together, these results strongly suggest that inhibition of PtdCho synthesis constitutes one of the primary events by which C-2-ceramide triggers apoptosis in cerebellar granule neurons. NeuroReport 11:3103-3108 (C) 2000 Lippincott Williams & Wilkins. |
| Databáze: | OpenAIRE |
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