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U današnje vrijeme sve više raste svijest o važnosti očuvanja zdravlja te postizanju istog kod oboljelih što je usko vezano uz zdravstveni sektor gdje se žele smanjiti kontaminacije uzrokovane različitim mikoorganizmima. U tu svrhu sve veći interes privlače antimikrobna svojstva tkanina primjenom kojih se može suzbiti razvoj i širenje opasnih mikrobnih infekcija koje uzrokuju bakterije poput Escherichie coli, Klebsielle pneumonia, Pseudomonas aeruginosa te Acinetobacter baumannii u bolničkom okruženju. Tkanine se mogu impregnirati različitim antimikrobnim agensima koji osim suzbijanja mikroorganizama ne smiju štetno djelovati na čovjeka. U ovom radu preliminarno je ispitana potencijalna toksičnost tvari kojima su impregnirane tekstilije namijenjene za uporabu u bolnicama na modelu kulture stanica - humanih keratinocita. Za određivanje proliferacije i vijabilnosti stanica korištena je MTT metoda nakon 72 h. Primijećen je utjecaj broja pranja na impregnirane tekstilije – što je tkanina više puta oprana pretpostavka je da na njoj zaostaje manje štetnih tvari što je potvrđeno manjim citotoksičnim učinkom na keratinocite. Metodom protočne citometrije određen je tip stanične smrti, odnosno udio vijabilnih, apoptotskih i nekrotičnih stanica u kulturi. Nowadays, the importance of maintaining as well as achieving health is increasing which is closely related to the health sector, where the contamination caused by different microorganisms needs to be reduced. Hence, the antimicrobial properties of textiles are being closely monitored due to their ability to suppress development and spreading of dangerous microbial infections caused by bacteria such as Escherichie coli, Klebsielle pneumonia, Pseudomonas aeruginosa and Acinetobacter baumannii in hospital setting. Textiles can be impregnated with various antimicrobial agents, which, in addition to suppressing microorganisms, must not have harmful effect on humans. In this study, the potential toxicity of textile impregnated substances intended for hospital use has been preliminary examined using the cell culture model of human keratinocytes. The MTT method after 72 h was used to determine cell proliferation and viability. Number of washes and its effect on the impregnated textiles has been observed – assumption is that the more the textile is being washed, the less of harmful substances will be left behind which is confirmed by the smaller cytotoxicity effect on keratinocytes. Flow cytometry was used to determine the type of cell death, that is, the proportion of viable, apoptotic and necrotic cells in culture. |
