Spatio-temporal dynamics of repetitive sequences in preimplantation rabbit embryos
| Autor: | Bonnet-Garnier, Amélie, Zichuan, Liu, Aguirre-Lavin, Tiphaine, VEREBNE TAR, Krisztina, Lehmann, Gaetan, Adenot, Pierre, Zhou, Qi, Dinnyes, Andras, Duranthon, Véronique, Beaujean Bobineau, Nathalie |
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| Přispěvatelé: | Biologie du développement et reproduction (BDR), Centre National de la Recherche Scientifique (CNRS)-École nationale vétérinaire d'Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA), State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences [Changchun Branch] (CAS), BioTalentum Ltd, EMBO. DEU., Biologie du Développement et Reproduction (BDR), Institut National de la Recherche Agronomique (INRA), École nationale vétérinaire d'Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS), University of Manchester [Manchester]. Manchester, GBR., ProdInra, Archive Ouverte, ProdInra, Migration |
| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: | |
| Zdroj: | EMBO conference Series on Nuclear Structure and Dynamics EMBO conference Series on Nuclear Structure and Dynamics, Sep 2011, L'Isle sur la Sorgue, France. 2011, EMBO Conference Series on Nuclear Structure and Dynamics 18th International Chromosome Conference 18. International Chromosome Conference 18. International Chromosome Conference, Aug 2011, Manchester, United Kingdom. 2011 18. International Chromosome Conference, Aug 2011, Manchester, United Kingdom. 2011, 18th International Chromosome Conference |
| Popis: | National audience; Nowadays, a critical question in the epigenetic field is how chromatin is organized within the cell nucleus and how chromatin architecture affects and even regulates gene expression. In this study we hypothesize that nuclear structure could be involved in the control of gene expression during early rabbit embryonic development. We focused on pericentric/centric heterochromatin, a peculiar region within nuclei, known to form higher-order chromatin structures. We therefore performed FISH (Fluorescent In Situ Hybridization) and immuno-FISH to evaluate the relative localization of two types of repetitive satellite sequences “Rsat” in preimplantation rabbit embryos, especially at the time of MET (Maternal to Embryonic-Transition). We found that pericentric heterochromatin dynamic changes during rabbit embryogenesis. After fertilization, the repetitive sequences are first dispersed within the nuclei of early stage embryos. Foci only progressively appeared in embryonic nuclei by the 8/16 cell stage, suggesting that dramatic changes of chromatin structure are associated to the progressive onset of transcription during MET. |
| Databáze: | OpenAIRE |
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