Lipid bilayer tethered inside a nanoporous support : a solid-state NMR investigation

Autor: Wattraint, O., Arnold, A., Auger, M., Bourdillon, C., Sarazin, C.
Přispěvatelé: Génie Enzymatique et Cellulaire (GEC), Université de Technologie de Compiègne (UTC)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)
Jazyk: angličtina
Rok vydání: 2005
Zdroj: Analytical Biochemistry
Analytical Biochemistry, Elsevier Masson, 2005, 336, pp.253-261
ISSN: 0003-2697
1096-0309
Popis: 31P and 1H solid-state nuclear magnetic resonance (NMR) experiments have been designed with the aim of studying directly the formation of supported bilayers tethered inside nanoporous aluminum oxide supports as a model of biomimetic membranes. The static and magic angle spinning 31P NMR spectra of the supported bilayers have been compared with the experimental and simulated spectra of a simpler model with cylindrical geometry, namely a phospholipid bilayer adsorbed on an oriented polymer sheet. The broadening observed for the nanoporous model is most likely due to the presence of paramagnetic ions in the aluminum oxide. A phospholipid lateral diffusion coefficient of (2.8 ± 0.4) × 10?8 cm2/s has been measured for the tethered bilayer on a spherical support, indicating a good fluidity as compared with adsorbed membrane models.
Databáze: OpenAIRE
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