Streptomyces rimosus GDS(L) Lipase: Production, Heterologous Overexpression and Structure-Stability Relationship

Autor:	Vujaklija, D., Abramić, M., Ivana Leščić Ašler, Maršić, T., Pigac, J.
Jazyk:	angličtina
Rok vydání:	2003
Předmět:	Streptomyces rimosus GDS(L) lipase lcsh:Food processing and manufacture lipase Streptomyces plate assay thermostability lcsh:TP368-456 lcsh:Biotechnology lcsh:TP248.13-248.65 mental disorders human activities behavioral disciplines and activities
Zdroj:	Food Technology and Biotechnology Volume 41 Issue 1 Food Technology and Biotechnology, Vol 41, Iss 1, Pp 89-93 (2003) Scopus-Elsevier
ISSN:	1334-2606 1330-9862
Popis:	Streptomyces rimosus lipase gene has been overexpressed in a heterologous host, S. lividans TK23. The maximal lipase activity was determined in the culture filtrates of the late stationary phase. Time course of lipase production was monitored by a modified plate assay. S. rimosus lipase gene has been located on the AseI B fragment approximately 2 Mb far from the left end of the S. rimosus linear chromosome. Out of eight examined streptomycetes, the presence of this rare type of bacterial lipase gene was detected in two belonging to the S. rimosus taxonomic cluster, and in one non-related species. Comparison of protein sequences of the Streptomyces lipolytic enzymes was performed. The result indicated the best structural stability of the putative S. coelicolor lipase-2.
Databáze:	OpenAIRE
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