Differential expression of interleukin-17 family cytokines in intact and complicated human atherosclerotic plaques

Autor: De Boer, Onno J., Van Der Meer, Jelger J., Teeling, Peter, Van Der Loos, Chris M., Idu, Mirza M., Van Maldegem, Febe, Aten, Jan, Van Der Wal, Allard C.
Přispěvatelé: ACS - Amsterdam Cardiovascular Sciences, Pathology, Surgery, AII - Amsterdam institute for Infection and Immunity
Jazyk: angličtina
Rok vydání: 2010
Zdroj: De Boer, O J, Van Der Meer, J J, Teeling, P, Van Der Loos, C M, Idu, M M, Van Maldegem, F, Aten, J & Van Der Wal, A C 2010, ' Differential expression of interleukin-17 family cytokines in intact and complicated human atherosclerotic plaques ', Journal of Pathology, vol. 220, no. 4, pp. 499-508 . https://doi.org/10.1002/path.2667
Journal of pathology, 220(4), 499-508. John Wiley and Sons Ltd
Journal of Pathology, 220(4), 499-508. John Wiley and Sons Ltd
ISSN: 0022-3417
DOI: 10.1002/path.2667
Popis: In addition to the classical TH1 and TH2 cytokines, members of the recently identified IL-17 cytokine family play an important role in regulating cellular and humoral immune responses. At present nothing is known about the role of these cytokines in atherosclerosis. Expression of IL-17A, -E and -F was investigated in atherosclerotic tissue by rtPCR and immunohistochemistry. IL-17E and its receptor were further studied in cultured smooth muscle cells and endothelial cells, using rtPCR and western blot. rtPCR showed that IL-17A, -E and -F were expressed in the majority of plaques under investigation. IL-17A/F was expressed by mast cells in all stages of plaque development. IL-17A/F+ neutrophils were always observed in complicated plaques, but hardly in intact lesions. IL-17A/F+ Tcells ('TH17') were never observed. IL-17E was expressed by smooth muscle cells and endothelial cells in both normal and atherosclerotic arteries, and in advanced plaques also extensively by mature B cells. Cultured smooth muscle cells and endothelial cells were found to express both IL-17E and its functional receptor (IL-17RB). The constitutive expression of IL-17E by resident plaque cells, and the additional presence of IL-17E(+) B cells and IL-17A/F+ neutrophils in advanced and complicated plaques indicates a complex contribution of IL-17 family cytokines in human atherosclerosis, depending on the stage and activity of the disease. Copyright (C) 2009 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd
Databáze: OpenAIRE
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