In vivo (13)C NMR determines metabolic fluxes and steady state in linseed embryos

Autor: Troufflard, S., Roscher, A., Thomasset, B., Barbotin, J.N., Rawsthorne, S., Portais, J.C.
Přispěvatelé: Génie Enzymatique et Cellulaire (GEC), Université de Technologie de Compiègne (UTC)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)
Jazyk: angličtina
Rok vydání: 2007
Zdroj: Phytochemistry
Phytochemistry, Elsevier, 2007, 68, pp.2341-2350
ISSN: 0031-9422
Popis: The dynamics of developing linseed embryo metabolism was investigated using 13C-labelling experiments where the real-time kinetics of label incorporation into metabolites was monitored in situ using in vivo NMR. The approach took advantage of the occurrence in this plant tissue of large metabolite pools – such as sucrose or lipids – to provide direct and quantitative measurement of the evolution of the labelling state within central metabolism. As a pre-requisite for the use of steady state flux measurements it was shown that isotopic steady state was reached within 3 h at the level of central intermediates whereas it took a further 6 h for the sucrose pool. Complete isotopic and metabolic steady state took 18 h to be reached. The data collected during the transient state where label was equilibrated but the metabolic steady state was incomplete, enabled the rates of lipid and sucrose synthesis to be measured in situ on the same sample. This approach is suitable to get a direct assessment of metabolic time-scales within living plant tissues and provides a valuable complement to steady state flux determinations.
Databáze: OpenAIRE
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