Periodate oxidized glucose oxidase@ZIF-8 nanocomposite
| Autor: | Ristić, Predrag, Stanišić, Marija, Đokić, Veljko, Balaž, Ana Marija, Mitić, Dragana, Prodanović, Radivoje, Todorović, Tamara |
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| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: | |
| Zdroj: | 19th International Conference on Nanosciences & Nanotechnologies, 5-8 July 2022, Thessaloniki, Greece Book of abstracts-NANOTEXNOLOGY NN22, 19th International Conference on Nanosciences & Nanotechnologies, 5-8 July 2022, Thessaloniki, Greece |
| Popis: | The durability of enzymes in harsh conditions can be enhanced by immobilization within metal-organic frameworks (MOFs) via a process called biomimetic mineralisation. Zeolitic imidazolate framework-8 (ZIF-8) is widely used as a protective coating to encapsulate proteins. The formation of nucleation centres and further biocomposite particle growth is entirely governed by the pure electrostatic interactions between the protein’s surface and positively charged Zn(II) metal ions. It was previously shown that enhancing these electrostatic interactions by a chemical modification of surface amino acid residues can lead to a rapid biocomposite formation. However, a chemical modification of carbohydrate components by periodate oxidation for glycoproteins can serve as an alternative strategy. In the present study, an industrially important enzyme glucose oxidase (GOx) was selected as a model system. Periodate oxidation of GOx by 2.5 mM sodium periodate increased negative charge on the enzyme molecule. Biomineralization experiments with oxidized GOx resulted in higher specific activity, effectiveness factor, and higher thermostability of the ZIF-8 biocomposites. Book of Abstracts |
| Databáze: | OpenAIRE |
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