Does the vertical vs horizontal positioning of surfaces affect either biofilm formation on different materials or their resistance to detachment?

Autor: Jha, Piyush-Kumar, Dallagi, Heni, Richard, Elodie, Deleplace, Maureen, Benezech, Thierry, Faille, Christine
Přispěvatelé: Université de Lille, CNRS, INRA, ENSCL, Unité Matériaux et Transformations - UMR 8207 [UMET], Unité Matériaux et Transformations (UMET) - UMR 8207, Institut Pasteur de Lille, Unité Matériaux et Transformations - UMR 8207 (UMET), Institut de Chimie du CNRS (INC)-Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS)-Université de Lille-Ecole Nationale Supérieure de Chimie de Lille (ENSCL), Plateformes Lilloises en Biologie et Santé - UMS 2014 - US 41 (PLBS), Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille)-Centre National de la Recherche Scientifique (CNRS), This work was supported by the ANR (Agence Nationale de la Recherche) for funding the FEFS project (contract number ANR-18-CE21-0010) and the region Hauts-de-France for funding the Interreg Veg-I-Tec project (Programme Interreg V France-Wallonia-Flanders, GoToS3)., Interreg Veg-I-Tec, ANR-18-CE21-0010,FEFS,La Mécanique des Fluides au Service de la Sécurité Alimentaire(2018), Centrale Lille-Institut de Chimie du CNRS (INC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Plateformes Lilloises en Biologie et Santé - UAR 2014 - US 41 (PLBS), Université de Lille, LillOA, APPEL À PROJETS GÉNÉRIQUE 2018 - La Mécanique des Fluides au Service de la Sécurité Alimentaire - - FEFS2018 - ANR-18-CE21-0010 - AAPG2018 - VALID, Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Supérieure de Chimie de Lille (ENSCL)-Institut de Chimie du CNRS (INC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)
Jazyk: angličtina
Rok vydání: 2022
Předmět:
Rinsing procedure
[SPI.GPROC] Engineering Sciences [physics]/Chemical and Process Engineering
Biofilm
Vertical/horizontal position
Material
Sedimentation
[SDV.IDA] Life Sciences [q-bio]/Food engineering
biochemical phenomena
metabolism
and nutrition
[PHYS.COND.CM-MS] Physics [physics]/Condensed Matter [cond-mat]/Materials Science [cond-mat.mtrl-sci]
[PHYS.COND.CM-SCM] Physics [physics]/Condensed Matter [cond-mat]/Soft Condensed Matter [cond-mat.soft]
[SDV.IDA]Life Sciences [q-bio]/Food engineering
[SDV.BBM] Life Sciences [q-bio]/Biochemistry
Molecular Biology
[PHYS.COND.CM-MS]Physics [physics]/Condensed Matter [cond-mat]/Materials Science [cond-mat.mtrl-sci]
[SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering
[SDV.BBM]Life Sciences [q-bio]/Biochemistry
Molecular Biology
[PHYS.COND.CM-SCM]Physics [physics]/Condensed Matter [cond-mat]/Soft Condensed Matter [cond-mat.soft]
Zdroj: Food Control
Food Control, Elsevier, 2022, Food Control, 133 (Part B), pp.108646. ⟨10.1016/j.foodcont.2021.108646⟩
Food Control, 2022, Food Control, 133 (Part B), pp.108646. ⟨10.1016/j.foodcont.2021.108646⟩
ISSN: 0956-7135
Popis: International audience; In the food industry, the control of surface hygiene is a major issue. It is therefore essential to identify those parameters that can affect the bacterial contamination of surfaces and the effectiveness of hygiene procedures. Numerous studies have focused on the role of bacteria, flow arrangements or materials, but almost nothing has been reported on the possible impact of vertical or horizontal positioning of surfaces on bacterial contamination. The aim of the current study was firstly to determine the ability of bacterial species usually found in food processing lines to form biofilms on surfaces positioned vertically or horizontally and then to assess the resistance of these biofilms to detachment. The experiments were carried out using three bacterial strains (Escherichia coli SS2, Bacillus cereus 98/4, and Pseudomonas fluorescens Pf1) that produced biofilms on glass, polypropylene and stainless steel (surface finishes 2B and 2R). We first observed that not only did the bacterial strain type impacts its ability to form biofilms (Ec-SS2 > Pf1 > Bc-98/4), but that the vertical vs horizontal position of the surface would also affect biofilm formation, probably due to the accumulation through sedimentation of bacteria on horizontal surfaces. However, the horizontally formed Pf1 biofilms were very fragile and could be partially removed by a gentle rinsing step. Lastly, no significant differences could be found in the ability to form biofilms on the different materials. The resistance to detachment to a standard rinsing process in a pilot rig was also investigated. While both strains and materials significantly affected the amount of biofilm detached, only Bc-98/4 biofilms were impacted by the surface position, with horizontal biofilms showing extreme resistance to shear forces. In conclusion, this study shows that horizontal surfaces in food environments probably represent an increased risk of contamination by bacteria frequently isolated from these environments and should be subjected to increased monitoring.
Databáze: OpenAIRE
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