Cell cycle regulated expression and phosphorylation of hpttg proto-oncogene product
| Autor: | Ramos-Morales, Francisco, Domínguez, África, Romero, Francisco, Luna, Rosa, Multon, Marie-Christine, Pintor-Toro, José Antonio |
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| Jazyk: | angličtina |
| Rok vydání: | 2000 |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname |
| ISSN: | 1476-5594 0950-9232 |
| Popis: | We recently isolated a cDNA for hpttg, the human homolog of rat pituitary tumor transforming gene. Now we have analysed the expression of hpttg as a function of cell proliferation. hPTTG protein level is up-regulated in rapidly proliferating cells, is down-regulated in response to serum starvation or cell confluence, and is regulated in a cell cycle-dependent manner, peaking in mitosis. In addition, we show that hPTTG is phosphorylated during mitosis. Immunodepletion and in vitro phosphorylation experiments, together with the use of a specific inhibitor, indicate that Cdc2 is the kinase that phosphorylates hPTTG. These results suggest that hpttg is induced by and may have a role in, regulatory pathways involved in the control of cell proliferation. |
| Databáze: | OpenAIRE |
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