Rats resistant to high-fat/high-sucrose diet-induced obesity and metabolic syndrome have a lower de novo lipogenesis in adipose tissues and liver
| Autor: | Jean-François Huneau, Dominique Hermier, Olivier Landry Mantha, Philippe Schmidely, Véronique Mathé, François Mariotti, Helene Fouillet |
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| Přispěvatelé: | Physiologie de la Nutrition et du Comportement Alimentaire (PNCA), AgroParisTech-Institut National de la Recherche Agronomique (INRA), Modélisation Systémique Appliquée aux Ruminants (MoSAR), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, ASN American Society for Nutrition. |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: | |
| Zdroj: | Nutrition 2018 Nutrition 2018, Jun 2018, Boston, United States HAL Nutrition 2018, ASN American Society for Nutrition., Jun 2018, Boston, United States |
| Popis: | International audience; Objectives There is a high variability of metabolic response to a high-fat/high-sucrose diet, with individuals being either resistant or prone to develop obesity (O) alone or associated with a metabolic syndrome (MS). This study aims to compare de novo lipogenesis (DNL) between rats prone or resistant to diet-induced O and MS. Methods Forty-two male Wistar rats were fed a high-fat/high-sucrose diet for 4 months. Body composition and metabolic parameters were measured at the end of the study and used to discriminate rats according to their sensitivity to O and MS, using unsupervised classification based on 3 markers of O (weight, total and visceral adiposities) and 3 markers of MS (HOMA-IR, plasma and liver triglycerides). DNL was measured in the liver, epididymal (EAT) and subcutaneous (SAT) adipose tissues by deuterium tracing: after an initial intraperitoneal load (20g per kg, 99.9 atom%), rats were exposed to D2O (5 atom%) in drinking water for 2 days prior to euthanasia, and D-enrichment was measured in tissue neutral lipids by EA-IRMS and in plasma by GC-MS. In each tissue, the net flux of lipids synthesized by DNL and stored in situ (net DNL, mg/d) was estimated using an equation derived from a precursor-product model. Results The classification distinguished 3 groups of rats: those resistant to O and MS (R, n=15), prone to O but resistant to MS (O, n=13), or prone to O and MS (OMS, n=14). The relative dietary intake per g of body weight was similar between the groups. Only OMS rats had steatosis (hepatic lipid content of 13% vs. 8% for R and O rats), and hepatic DNL was 40% lower in R and O rats compared to OMS. In adipose tissues, DNL was 35% (SAT) at 45% (EAT) lower in R rats compared to O, and 35% lower in O rats compared to OMS. Net DNL (mg/d) Liver EAT SAT R (n=15) 45.8 ± 14.3a 18.3 ± 4.1a 40.0 ± 13.4a O (n=13) 47.1 ± 13.7a 32.8 ± 12.6b 60.9 ± 20.6b OMS (n=14) 74.8 ± 15.8b 48.9 ± 8.2c 91.9 ± 22.0c Mean ± standard deviations. Different letters indicate differences between groups (P |
| Databáze: | OpenAIRE |
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