(2002). An 8 SCR long CD46CD[55-46] chimeric receptor acts as an inhibitor of both CD46 (MCP) and CD150 (SLAM) mediated cell-cell fusion induced by CD46-interacting Measles virus
| Autor: | D De Sousa Er Loveland B Kyriakou P Lanteri M Wild Ft Gerlier D., Christiansen |
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| Přispěvatelé: | Rollin, Bertrand, Virologie et pathogenèse virale (VPV), Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon, Immunite et Vaccination, Institut National de la Santé et de la Recherche Médicale (INSERM), The Austin Research Institute (THE AUSTIN RESEARCH INSTITUTE), Heidelberg University, This work was supported in part by grants from CNRS, and the Ministère de l'Education Nationale et de la Recherche et de la Technologie (PRFMMIP) (D.G.) and the NHMRC (Australia) (B.L., P.K., M.L.). D.C. was supported by a Marie Curie EU Fellowship and a Fondation pour la Recherche Médicale Fellowship. |
| Jazyk: | angličtina |
| Rok vydání: | 2006 |
| Předmět: |
[SDV.MP.VIR] Life Sciences [q-bio]/Microbiology and Parasitology/Virology
viruses Recombinant Fusion Proteins/chemistry/*physiology Molecular Sequence Data Immunoglobulins CHO Cells Research Support Membrane Fusion female genital diseases and pregnancy complications Measles virus/*physiology Repetitive Sequences Amino Acid Cricetinae Glycoproteins/*antagonists & inhibitors [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology Animals Amino Acid Sequence Antigens Membrane Glycoproteins/antagonists & inhibitors/chemistry/*physiology OCIS 000.1430 CD/chemistry/*physiology Non-U.S. Gov't CD46 |
| Popis: | According to their cellular receptor use, measles virus (MV) strains can be separated into two phenotypes, CD46-using and CD46-non-using. A long chimeric receptor, CD46CD[55-46], was generated from the CD46 backbone, encompassing the four short consensus repeat (SCR) domains of CD46 linked via a flexible glycine hinge to SCR1 and SCR2 of CD55, SCR3 and SCR4 of CD46 and the STP, transmembrane and cytoplasmic tail of CD46. This chimeric receptor was proficient for MV binding but deficient in mediating MV-induced cell-to-cell fusion and virus replication, possibly due to the extended distance between the MV haemagglutinin (H) binding site (CD46 SCR1-SCR2) and the cell membrane. When coexpressed with either wild-type CD46 or CD150, this fusion-incompetent receptor exerted a dominant negative effect and inhibited both cell-to-cell fusion and entry of MV with CD46-using, but not CD46-non-using, phenotype. A soluble octameric CD46-C4bpalpha exhibited similar CD46- and CD150-mediated fusion inhibition properties only against CD46-using MV. This suggests that the long CD46CD[55-46] receptor acts by sequestering incoming MV prior to its binding to the shorter functional CD46 or CD150 receptor. |
| Databáze: | OpenAIRE |
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