Amino acids attached to 2'-amino-LNA: Synthesis of DNA mixmer oligonucleotides with increased duplex stability
| Autor: | Marie Willaing Johannsen, Jesper Wengel, Michael Chr. Wamberg, Lia Crispino, Neerja Kalra |
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| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Zdroj: | Johannsen, M W, Wengel, J, Wamberg, M C, Crispino, L & Kalra, N 2010, ' Amino acids attached to 2'-amino-LNA: Synthesis of DNA mixmer oligonucleotides with increased duplex stability ' . CIÊNCIAVITAE University of Southern Denmark |
| Popis: | The synthesis of 2'-amino-LNA (locked nucleic acid) opens up exciting possibilities for modification of nucleic acids by conjugation to the 2'-nitrogen. Incorporation of unmodified and N-functionalized 2'-amino-LNA nucleotides improve duplex stability compared to unmodified DNA. 2'-Amino-LNA nucleosides derivatized with amino acids have been synthesized and incorporated into DNA oligonucleotides. Following oligonucleotide synthesis, peptides have been added using solid phase peptide coupling chem. Modification of oligonucleotides with pos. charged residues greatly improves thermal stability. |
| Databáze: | OpenAIRE |
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