The environmental Acinetobacter baumannii isolate DSM30011 reveals clues into the preantibiotic era genome diversity, virulence potential, and niche range of a predominant nosocomial pathogen

Autor:	Repizo, Guillermo D., Viale, Alejandro M., Borges, Vítor, Cameranesi, María M., Taib, Najwa, Espariz, Martín, Brochier-Armanet, Céline, Gomes, João Paulo, Salcedo, Suzana P.
Jazyk:	angličtina
Rok vydání:	2017
Předmět:	Acinetobacter baumannii Infecções Respiratórias purl.org/becyt/ford/1 [https] COMPARATIVE GENOMICS PREANTIBIOTIC ERA BACTERIUM Ciencias Biológicas CRISPRS/CAS Otras Ciencias Biológicas purl.org/becyt/ford/1.6 [https] VIRULENCE FACTORS CIENCIAS NATURALES Y EXACTAS
Zdroj:	CONICET Digital (CONICET) Consejo Nacional de Investigaciones Científicas y Técnicas instacron:CONICET
Popis:	Acinetobacter baumannii represents nowadays an important nosocomial opportunistic pathogen whose reservoirs outside the clinical setting are obscure. Here, we traced the origins of the collection strain A. Baumannii DSM30011 to an isolate first reported in 1944, obtained from the enriched microbiota responsible of the aerobic decomposition of the resinous desert shrub guayule. Whole-genome sequencing and phylogenetic analysis based on core genes confirmed DSM30011 affiliation to A. Baumannii. Comparative studies with 32 complete A. Baumannii genomes revealed the presence of 12 unique accessory chromosomal regions in DSM30011 including five encompassing phage-related genes, five containing toxin genes of the type-6 secretion system, and one with an atypical CRISPRs/cas cluster. No antimicrobial resistance islands were identified in DSM30011 agreeing with a general antimicrobial susceptibility phenotype including folate synthesis inhibitors. The marginal ampicillin resistance of DSM30011 most likely derived from chromosomal ADC-type ampC and blaOXA-51-type genes. Searching for catabolic pathways genes revealed several clusters involved in the degradation of plant defenses including woody tissues and a previously unreported atu locus responsible of aliphatic terpenes degradation, thus suggesting that resinous plants may provide an effective niche for this organism. DSM30011 also harbored most genes and regulatory mechanisms linked to persistence and virulence in pathogenic Acinetobacter species. This strain thus revealed important clues into the genomic diversity, virulence potential, and niche ranges of the preantibiotic era A. Baumannii population, and may provide an useful tool for our understanding of the processes that led to the recent evolution of this species toward an opportunistic pathogen of humans. Fil: Repizo, Guillermo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina. Centre National de la Recherche Scientifique; Francia Fil: Viale, Alejandro Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina Fil: Borges, Vitor. National Institute of Health. Department Of Infectious Diseases. Bioinformatics Unit; Portugal Fil: Cameranesi, María Marcela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina Fil: Taib, Najwa. Centre National de la Recherche Scientifique; Francia. Université Claude Bernard Lyon 1; Francia Fil: Espariz, Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina Fil: Brochier Armanet, Celine. Université Claude Bernard Lyon 1; Francia Fil: Gomes, João Paulo. National Institute of Health. Department Of Infectious Diseases. Bioinformatics Unit; Portugal Fil: Salcedo, Suzana P.. Centre National de la Recherche Scientifique; Francia
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=dedup_wf_001::bb5a0e96a7bae3d35f01829c8e557c6c https://academic.oup.com/gbe/article/9/9/2292/4095374 Zobrazit plný text záznamu