Prrx1 controls endodermal organ laterality in vertebrates
| Autor: | Ocaña, Oscar H., Coskun, Hakan, Castroviejo, Noemi, Galcerán, Joan, Nieto, M. Ángela |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: | |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname |
| Popis: | Resumen del póster presentado al European Developmental Biology Congress (EDBC), celebrado en Alicante del 23 al 26 de octubre de 2019. The establishment of left-right asymmetries is crucial during embryonic development for the proper positioning and morphogenesis of internal organs. A transient L/R asymmetric expression of Prrx1 in the lateral plate mesoderm (LMP) leads to L/R asymmetric cell movements that displace the posterior pole of the heart to the left, promoting its normal position. We have now examined whether a similar mechanism in also involved in the lateralization of endodermal organs. We show here that prrx1a “crispant” zebrafish embryos present gut looping defects together with abnormal positioning of the liver and pancreas all remaining at their initial position in the midline. A prrx1a expression reporter line shows that LPM cells descendants from prrx1a+ cells have a L/R asymmetric pattern of migration. Cells from the right LPM move ventrally as previously described and push the gut to left. In prrx1a crispants, this migratory pathway if defective, disturbing not only the displacement of the gut from the midline but also affecting the cellular organisation and morphological asymmetry of the LPM. We also show that this mechanism is conserved in chick embryo. Thus, we propose that, similarly to the situation in the heart, a differential L/R EMT regulates the epithelial and migratory properties of the LPM and drives endodermal organ laterality in vertebrates. |
| Databáze: | OpenAIRE |
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