The microRNA miR7695 is involved in the rice immune response to pathogen infection
| Autor: | Sánchez-Sanuy, Ferran, Tomiyama, Shiho, Okada, Kazunori, Hsing, Yue-Ie, San Segundo, Blanca, Campo, Sonia |
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| Rok vydání: | 2019 |
| Předmět: | |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname |
| Popis: | Resumen del póster presentado al Congreso 'At the Forefront of Plant Research', celebrado en Barcelona (España) del 6 al 8 de mayo de 2019. MicroRNAs (miRNAs) are short regulatory non-coding RNAs that guide gene silencing by triggering sequence-specific cleavage or translational repression of target transcripts. In plants, miRNAs emerged as versatile regulators of gene expression in developmental processes and adaptive responses to environmental stresses, including pathogen infection. Certain plant miRNAs have been shown to function in cross-kingdom regulation of gene expression (i.e. miR159 and miR166 can move from cotton plants to the fungal pathogen Verticillium dahliae for specific silencing of pathogen virulence genes). In rice, the fungus Magnaporthe oryzae is the causal agent of the rice blast disease, one of the most devastating fungal diseases of cultivated rice worldwide. Although a plethora of rice miRNAs have been shown to be regulated during M. oryzae infection, their biological function remains largely unknown. Here, we report that a miRNA, miR7695, contributes to rice immunity. MiR7695 targets an alternatively spliced transcript of the Natural resistance-associated macrophage protein 6 (OsNramp6) gene encoding an iron transporter from rice. Activation-tagged MIR7695 rice plants (MIR7695-Ac) exhibited enhanced resistance to M. oryzae infection. RNA-seq analysis revealed that blast resistance in MIR7695-Ac plants is associated with stronger induction of defense-related genes, including pathogenesis-related and diterpenoid biosynthetic genes. Furthermore, rice plants grown under high iron supply showed blast resistance, indicating that iron is a factor in controlling blast resistance. During pathogen infection, iron accumulates in the vicinity of fungal aspersoria, the sites of pathogen entry, and in cells surrounding infected regions of the rice leaf. This observation supports that rice plants use strategies to locally increase Fe content to prevent penetration and spread of the pathogen into the leaf tissue. A better understanding of the mechanisms that are regulated by miR7695 during rice immunity and crosstalk with iron homeostasis will help in designing novel strategies to control rice blast disease. |
| Databáze: | OpenAIRE |
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