Stabilnost i toksični učinci specija arsena u uzorcima voda
| Autor: | Alispahić, Amra |
|---|---|
| Přispěvatelé: | Krivohlavek, Adela, Galić, Nives |
| Jazyk: | chorvatština |
| Rok vydání: | 2022 |
| Předmět: |
ecotoxicity
water stability investigation arsenic species PRIRODNE ZNANOSTI. Kemija NATURAL SCIENCES. Chemistry ekotoksičnost stabilnost UHPLC–ICPMS– voltammetry voltametrija specije arsena voda cytotoxicity citotoksičnost udc:54(043.3) UHPLC–ICP–MS citotoksičnost/ ekotoksičnost/ specije arsena/ stabilnost/ voda/ voltametrija/ UHPLC–ICP–MS Kemija. Kristalografija. Mineralogija Chemistry. Crystallography. Mineralogy |
| Popis: | U ovom radu ispitana je stabilnost omjera anorganskih specija arsena As(III)/As(V) u modelnim otopinama i uzorcima prirodnih podzemnih voda s četiri lokacije u Hrvatskoj uz dodatak sljedećih konzervansa: limunska kiselina, natrijev citrat, kalijev oksalat, kalijev–natrijev tartarat i octena kiselina. Specijacijska analiza provedena je metodom voltametrije anodnog otapanja i UPLC–ICP–MS. Utvrđeno je kako kombinacija ispitanih konzervansa s octenom kiselinom uspješno stabilizira omjer specija arsena te je on u modelnim otopinama stabilan do 8 dana, dok je u uzorcima prirodnih voda stabilan 6 do 7 dana. Akutnim testom ekotoksičnosti određena je vrijednost LC50 nakon 24 h na Daphnia magna. Dobivene vrijednosti su 5,07 ppm za As(III), 10,27 ppm za As(V) i 0,58 ppm za As(III) s nanočesticama željeza. MTT testom citotoksičnosti na HepG2 staničnoj liniji određena je vrijednosti LC50 nakon 24 h. Najveća citotoksičnost određena je za As(III) uz dodatak SPIO nanočestica, dok As(V) ne inducira staničnu smrt. In this paper, the stability of inorganic arsenic species ratio As(III)/As(V) in model solutions and natural groundwater samples from four locations in Croatia was investigated. Selected preservatives were citric acid, sodium citrate, potassium oxalate, potassium sodium tartrate and acetic acid. Speciation analysis was performed by anodic stripping voltammetry method and UPLC–ICP–MS. It was shown that the combination of the tested preservatives with acetic acid stabilize the arsenic species ratio for up to 8 days in model solutions and for 6 to 7 days in natural water samples. The LC50 values determined by acute ecotoxicity test on Daphnia magna after 24 h amounted to 5.07, 10.27 and 0.58 ppm for As(III), As(V), As(III)/iron nanoparticles, respectively. Cytotoxicity was determined by MTT test on human hepatocarcinoma cell line (HepG2) after 24 h and expressed as LC50 value. The most pronounced effect was observed for As(III)/Fe nanoparticles, while As(V) was inactive. |
| Databáze: | OpenAIRE |
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