A routine method to determine the chlorophyll a, pheophytin a and B-carotene contents of isolated photosystem II reaction center complexes

Autor: Eijckelhoff, C., Dekker, J.P.
Přispěvatelé: Biophysics Photosynthesis/Energy
Jazyk: angličtina
Rok vydání: 1997
Předmět:
Zdroj: Photosynthesis Research, 52, 69-73. Springer Netherlands
Eijckelhoff, C & Dekker, J P 1997, ' A routine method to determine the chlorophyll a, pheophytin a and B-carotene contents of isolated photosystem II reaction center complexes. ', Photosynthesis Research, vol. 52, pp. 69-73 . https://doi.org/10.1023/A:1005834006985
ISSN: 0166-8595
DOI: 10.1023/a:1005834006985
Popis: The most simple way in which the stoichiometry of chlorophyll a, pheophytin a and ̄-carotene in isolated Photosystem II reaction center complexes can be determined is by analysis of the spectrum of the extracted pigments in 80% acetone. We present two different calculation methods using the extinction coefficients of the purified pigments in 80% acetone at different wavelengths. One of these methods also accounts for the possible presence of chlorophyll b. The results are compared with results obtained with HPLC pigment analysis, and indicate that these methods are suitable for routine determination of the pigment stoichiometry of isolated Photosystem II reaction center complexes.
Databáze: OpenAIRE
Externí odkaz: