| Autor: |
Pérals, K, Capiaux, H, Vincourt, JB, Louarn, JM, Sherratt, DJ, Cornet, F |
| Jazyk: |
angličtina |
| Rok vydání: |
2016 |
| Předmět: |
|
| DOI: |
10.1046/j.1365-2958.2001.02277.x |
| Popis: |
Chromosome dimers form in bacteria by recombination between circular chromosomes. Resolution of dimers is a highly integrated process involving recombination between dif sites catalysed by the XerCD recombinase, cell division and the integrity of the division septum-associated FtsK protein and the presence of dif inside a restricted region of the chromosome terminus, the dif activity zone (DAZ). We analyse here how these phenomena collaborate. We show that (i) both inter- and intrachromosomal recombination between dif sites are activated by their presence inside the DAZ; (ii) the DAZ-specific activation only occurs in conditions supporting the formation of chromosome dimers; (iii) overexpression of FtsK leads to a general increase in dif recombination irrespective of dif location; (iv) overexpression of FtsK does not improve the ability of dif sites inserted outside the DAZ to resolve chromosome dimers. Our results suggest that the formation of an active XerCD-FtsK-dif complex is restricted to when a dimer is present, the features of chromosome organization that determine the DAZ playing a central role in this control. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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