The role of axonal Kv1 channels in CA3 pyramidal cell excitability

Autor: Rama, Sylvain, Zbili, Mickael, Fékété, Aurélie, Tapia, Mónica, Benitez, Maria Jose, Boumedine, Norah, Garrido, Juan Jose, Debanne, Dominique, Garrido, Juan, Benitez, Maria
Přispěvatelé: Epilepsie et ischémie cérébrale, Université de la Méditerranée - Aix-Marseille 2-Institut National de la Santé et de la Recherche Médicale (INSERM), Unité de Neurobiologie des canaux Ioniques et de la Synapse (UNIS - Inserm U1072), Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Universidad Autonoma de Madrid (UAM), Department of Genetics, Genomics and Animal Breeding Group, University of Córdoba [Córdoba], Universidad Autónoma de Madrid (UAM), Universidad de Córdoba = University of Córdoba [Córdoba]
Jazyk: angličtina
Rok vydání: 2017
Předmět:
Zdroj: Scientific Reports
Scientific Reports, Nature Publishing Group, 2017, 7 (1), ⟨10.1038/s41598-017-00388-1⟩
Scientific Reports, 2017, 7 (1), ⟨10.1038/s41598-017-00388-1⟩
ISSN: 2045-2322
DOI: 10.1038/s41598-017-00388-1⟩
Popis: International audience; Axonal ion channels control spike initiation and propagation along the axon and determine action potential waveform. We show here that functional suppression of axonal Kv1 channels with local puff of dendrotoxin (DTx), laser or mechanical axotomy significantly increased excitability measured in the cell body. Importantly, the functional effect of DTx puffing or axotomy was not limited to the axon initial segment but was also seen on axon collaterals. In contrast, no effects were observed when DTx was puffed on single apical dendrites or after single dendrotomy. A simple model with Kv1 located in the axon reproduced the experimental observations and showed that the distance at which the effects of axon collateral cuts are seen depends on the axon space constant. In conclusion, Kv1 channels located in the axon proper greatly participate in intrinsic excitability of CA3 pyramidal neurons. This finding stresses the importance of the axonal compartment in the regulation of intrinsic neuronal excitability.
Databáze: OpenAIRE
Externí odkaz: