Molecular cloning and mammalian expression of human beta 2-glycoprotein I cDNA

Autor:	Kristensen, Torsten, Schousboe, Inger, Boel, Espen, Mulvihill , Eileen M, Hansen, Rita Rosendahl, Møller, Karin Bach, Møller, Niels Peter Hundahl, Sottrup-Jensen, Lars
Jazyk:	angličtina
Rok vydání:	1991
Zdroj:	Kristensen, T, Schousboe, I, Boel, E, Mulvihill, E M, Hansen, R R, Møller, K B, Møller, N P H & Sottrup-Jensen, L 1991, ' Molecular cloning and mammalian expression of human beta 2-glycoprotein I cDNA. ', FEBS Letters, vol. 289, no. 2, pp. 183-186 .
Popis:	Human β2-glycoprotein (β2gpI) cDNA was isolated from a liver cDNA library and sequenced. The cDNA encoded a 19-residue hydrophobic signal peptide followed by the mature β2gpI of 326 amino acid residues. In liver and in the hepatoma cell line HepG2 there are two mRNA species of about 1.4 and 4.3 kb, respectively, hybridizing specifically with the β2gpI cDNA. Upon isoelectric focusing, recombinant β2gpI obtained from expression of β2gpI cDNA in baby hamster kidney cells showed the same pattern of bands as β2gpI isolated from plasma, and at least 5 polypeptides were visible
Databáze:	OpenAIRE
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