| Zdroj: |
Jørgensen, V R, Dueholm, M S, Knutsson, S, Nierychlo, M A, Kristensen, J M, Petriglieri, F, Dottorini, G, Yashiro, E, Karst, S M, Albertsen, M & Nielsen, P H 2019, ' Global reference database of microbes in anaerobic digesters ', IWC-16th World Conference on Anaerobic Digestion, Delft, Netherlands, 24/06/2019-27/06/2019 . |
| Popis: |
In this study, we aim to establish an anaerobic digester-specific reference database, comprised of full-length microbial 16S rRNA gene sequences from anaerobic digesters across the world (the Global Database of Microbes in Anaerobic Digesters, www.midasfieldguide.org/en/global-database-of-microbes/). The project utilizes a novel high-throughput, primer-free sequencing method to reveal the true microbial composition without being skewed by primer choice. The database is based on samples from more than 25 countries, making it the largest of its kind to date. The included digester types cover various process designs and a range of substrate feed compositions (with a primary focus is wastewater sludge). Here we present an overview of procedures for sampling, sample handling, sequencing, and the improved 16S rRNA gene reference database and taxonomy. |
