Natural hypolignification is associated with extensive oligolignol accumulation in flax stems
| Autor: | Rudy Huis, Kris Morreel, Ophelie Fliniaux, Anca Lucau, Stephane Fenart, Sébastien Grec, Godfrey Neutelings, Brigitte Chabbert, François Mesnard, Simon Hawkins |
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| Přispěvatelé: | Stress Abiotiques et Différenciation des Végétaux Cultivés (SADV), Institut National de la Recherche Agronomique (INRA)-Université de Lille, Sciences et Technologies, Universiteit Gent = Ghent University [Belgium] (UGENT), Université de Picardie Jules Verne (UPJV), Fractionnement des AgroRessources et Environnement (FARE), Université de Reims Champagne-Ardenne (URCA)-Institut National de la Recherche Agronomique (INRA) |
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
lignans
phenols transcription factors lignin laccase peroxidase [SDV]Life Sciences [q-bio] lignine [SDV.IDA]Life Sciences [q-bio]/Food engineering [SDE]Environmental Sciences [SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering facteur de transcription métabolisme expression des gènes |
| Zdroj: | Plant Physiology Plant Physiology, American Society of Plant Biologists, 2012, 158 (4), pp.1893-1915 HAL Plant Physiology 4 (158), 1893-1915. (2012) |
| ISSN: | 0032-0889 1532-2548 |
| Popis: | International audience; Flax (Linum usitatissimum) stems contain cells showing contrasting cell wall structure: lignified in inner stem xylem tissue and hypolignified in outer stem bast fibers. We hypothesized that stem hypolignification should be associated with extensive phenolic accumulation and used metabolomics and transcriptomics to characterize these two tissues. 1H nuclear magnetic resonance clearly distinguished inner and outer stem tissues and identified different primary and secondary metabolites, including coniferin and p-coumaryl alcohol glucoside. Ultrahigh-performance liquid chromatography-Fourier transform ion cyclotron resonance-mass spectrometry aromatic profiling (lignomics) identified 81 phenolic compounds, of which 65 were identified, to our knowledge, for the first time in flax and 11 for the first time in higher plants. Both aglycone forms and glycosides of monolignols, lignin oligomers, and (neo)lignans were identified in both inner and outer stem tissues, with a preponderance of glycosides in the hypolignified outer stem, indicating the existence of a complex monolignol metabolism. The presence of coniferin-containing secondary metabolites suggested that coniferyl alcohol, in addition to being used in lignin and (neo)lignan formation, was also utilized in a third, partially uncharacterized metabolic pathway. Hypolignification of bast fibers in outer stem tissues was correlated with the low transcript abundance of monolignol biosynthetic genes, laccase genes, and certain peroxidase genes, suggesting that flax hypolignification is transcriptionally regulated. Transcripts of the key lignan genes Pinoresinol-Lariciresinol Reductase and Phenylcoumaran Benzylic Ether Reductase were also highly abundant in flax inner stem tissues. Expression profiling allowed the identification of NAC (NAM, ATAF1/2, CUC2) and MYB transcription factors that are likely involved in regulating both monolignol production and polymerization as well as (neo)lignan production. |
| Databáze: | OpenAIRE |
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