| Autor: |
Pawlowski, Jan, Esling, Philippe, Lejzerowicz, Franck, Visco, Joana A., Cedhagen, Tomas |
| Jazyk: |
angličtina |
| Rok vydání: |
2015 |
| Zdroj: |
Pawlowski, J, Esling, P, Lejzerowicz, F, Visco, J A & Cedhagen, T 2015, Next generation sequencing assays for benthic monitoring of the environmental impact associated with salmon farming (pilot study) . |
| Popis: |
This report presents the study of foraminiferal and metazoan benthic community based on next-generation sequencing (NGS) of environmental DNA and RNA (eDNA/RNA). The objective of this study was to test the application of NGS assays for benthic monitoring of salmon farms in Norway, in order to overcome the limitations of traditional morphology-based approach.We analysed 140 samples of eDNA/RNA extracted from surface sediment samples collected at 4 salmon farming sites in Norway. We sequenced the variable region 37F of 18S rRNA gene specific to foraminifera, and the variable region V4 of the 18S rRNA gene to assess the diversity of metazoans. We compared our data to the results of macrofaunal surveys of the same sites and we tested the congruence of benthic indices and the assessment of ecological quality inferred from NGS and morphological data.The results of our study provide strong evidence for the usefulness of NGS approach to assess the benthic diversity of foraminifera and metazoans. For both groups we found a good correlation between diversity indices inferred from NGS and morphological data. The foraminifera show a clear pattern of the diversity increase with the distance to cages. The NGS metazoan data reflect the same environmental changes as morphology-based inventory of macrofauna, despite the differences in taxonomic composition of both assemblages. In conclusion, our report indicates that the NGS assays provide an assessment of the ecological quality equivalent to the one provided by morphological analyses. Although the further validation studies are necessary to improve the accuracy of NGS data interpretation, in view of our results the NGS metabarcoding approach appears as a promising alternative to the classical morpho-taxonomic surveys. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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