Migration of human antigen-presenting cells in a human skin graft onto nude mice model after contact sensitization
| Autor: | Hoefakker, S., Balk, H.P., Boersma, W.J.A., Joost, T. van, Notten, W.R.F., Claassen, E. |
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| Přispěvatelé: | TNO Preventie en Gezondheid |
| Jazyk: | angličtina |
| Rok vydání: | 1995 |
| Předmět: |
Adult
Contact allergen Time Factors Mouse skin transplantation Transplantation Heterologous HLA DR antigen Dermatitis Contact Immunoenzyme Techniques Mice Interleukin 1alpha Cell Movement Animal model Cell migration Animal experiment Biology Mice Inbred BALB C Antigen presenting cell Rhodamine b Tumor necrosis factor alpha Animal Xenograft Interleukin 1beta Dendritic Cells Nonhuman Immunohistochemistry Cytokines Lymph node Female Lymph Nodes Epidermis Controlled study Dendritic cell Keratinocyte Human |
| Zdroj: | Immunology, 2, 86, 296-303 |
| Popis: | Fluorescent contact chemical allergens provoke sensitization after application on both syngeneic and allogeneic skin grafts in mice. We attempted to determine whether the functional activity in a contact sensitization response of human skin graft was affected at the level of antigen uptake and migration. After xenogeneic skin transplantation, we examined the effect of topical exposure of the graft to rhodamine B isothiocyanate (RITC). This paper describes the migration of RITC-carrying cells and human major histocompatibility complex (MHC) class II DR (HLA-DR)+ cells, from the graft to mouse draining lymph nodes. As demonstrated by immunohistochemistry, grafting resulted in a time-dependent decrease of human HLA-DR+ and CD1a+ cells, and an increase of mouse MHC class II (Ia)+ cells within the graft. Application of RITC on a 3-week-old human skin graft showed optimal migration capability compared to 6- or 9-week-old grafts. In addition, the time-dependent increase of frequencies of RITC+ and HLA-DR+ cells in the draining lymph nodes, and the time-dependent decrease of HLA-DR+ cells in the 3-week-old human skin graft, were concurrent. Supporting these data, human cytokine interleukin-1α (IL-1α), IL-1β and tumour necrosis factor-α (TNF-α), analysis in situ revealed that cytokine production by keratinocytes, a property associated with dendritic cell migration, was preserved in the human skin graft. Thus, like dendritic cells in contact sensitization in allografted skin, dendritic cells from human xenografted skin onto nude mice are capable of migration to mouse draining lymph nodes after allergen application. Induction of contact hypersensitivity is possible in a human skin graft onto nude mice model, although the use of this ex vivo model to analyze contact sensitivity is probably limited to 3 weeks after transplantation. Chemicals/CAS: rhodamine B, 81-88-9; Cytokines |
| Databáze: | OpenAIRE |
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