14-3-3 proteins interact with a 13-lipoxygenase, but not with a 9-lipoxygenase
| Autor: | Holtman, W.L., Roberts, M.R., Oppedijk, B.J., Testerink, C., Zeijl, M.J. van, Wang, M. |
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| Jazyk: | angličtina |
| Rok vydání: | 2000 |
| Předmět: |
Interaction
Tyrosine 3-Monooxygenase 13 lipoxygenase Lipoxygenase enzyme purification immunoprecipitation Western blotting Hordeum vulgare subsp. vulgare Escherichia coli Immunosorbent Techniques enzyme substrate complex Chemistry Physical food and beverages barley Antibodies Monoclonal Proteins nucleotide sequence Hordeum Surface Plasmon Resonance protein family Recombinant Proteins unclassified drug protein phosphorylation Isoenzymes germination 14-3-3 Proteins 9 lipoxygenase |
| Zdroj: | FEBS Letters, 1, 474, 48-52 |
| Popis: | Associations between lipoxygenases (Lox) and 14-3-3 proteins were demonstrated by two different methods. First, immunoprecipitation experiments, using isoenzyme-specific monoclonal Lox antibodies, showed that 14-3-3 proteins co-precipitate with 13-Lox, but not with the 9-Lox from barley. Second, interactions between 13-Lox and 14-3-3 were established by surface plasmon resonance studies, showing that 13-Lox binds with 14-3-3 proteins in a concentration-dependent manner. The interactions between 14-3-3 proteins and 13-Lox may reveal their role during plant development. Copyright (C) 2000 Federation of European Biochemical Societies. Molecular Sequence Numbers: GENBANK: L35931, L37358, X62388, X93170, Y14200; Chemicals/CAS: 13-lipoxygenase, EC 1.13.11.-; 14-3-3 Proteins; Antibodies, Monoclonal; Isoenzymes; Lipoxygenase, EC 1.13.11.12; Proteins; Recombinant Proteins; Tyrosine 3-Monooxygenase, EC 1.14.16.2 |
| Databáze: | OpenAIRE |
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