Characterisation and in vitro and in vivo evaluation of supercritical-CO2-foamed β-TCP/PLCL composites for bone applications
| Autor: | Pitkänen, S, Paakinaho, K, Pihlman, H, Ahola, N, Hannula, M, Asikainen, S, Manninen, M, Morelius, M, Keränen, P, Hyttinen, J, Kellomäki, M, Laitinen-Vapaavuori, O, Miettinen, S |
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| Přispěvatelé: | Tampere University, BioMediTech, Lääketieteen ja terveysteknologian tiedekunta - Faculty of Medicine and Health Technology, University of Helsinki, Department of Chemical and Metallurgical Engineering, Orton Foundation, Aalto-yliopisto, Aalto University, Equine and Small Animal Medicine, Helsinki One Health (HOH), Outi Vapaavuori / Principal Investigator, Softis - pehmytkudossairaudet, Petbone – ortopedia, fysioterapia, kivunlievitys |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
lcsh:Diseases of the musculoskeletal system
beta-tricalciumphosphate lcsh:Surgery osteogenic differentiation rabbit distal femur defect Composite 413 Veterinary science SCAFFOLDS Poly(L-lactide-co-ε-caprolactone) poly(L-lactide-co-epsilon-caprolactone) POLY-L-LACTIDE REGENERATION Osteogenic differentiation Lääketieteen bioteknologia - Medical biotechnology Bone substitute composite Rabbit distal femur defect ADIPOSE STEM-CELLS 318 Medical biotechnology β-tricalciumphosphate ENHANCE lcsh:RD1-811 217 Medical engineering DEGRADATION Adipose stem cells adipose stem cells poly(L-lactide-co-ε-caprolactone) STROMAL CELLS TISSUE 216 Materials engineering 1182 Biochemistry cell and molecular biology lcsh:RC925-935 |
| Zdroj: | European Cells & Materials, Vol 38, Pp 35-50 (2019) |
| Popis: | Most synthetic bone grafts are either hard and brittle ceramics or paste-like materials that differ in applicability from the gold standard autologous bone graft, which restricts their widespread use. Therefore, the aim of the study was to develop an elastic, highly porous and biodegradable beta-tricalciumphosphate/poly(L-lactide-co-epsilon-caprolactone) (beta-TCP/PLCL) composite for bone applications using supercritical CO2 foaming. Ability to support osteogenic differentiation was tested in human adipose stem cell (hASC) culture for 21 d. Biocompatibility was evaluated for 24 weeks in a rabbit femur-defect model. Foamed composites had a high ceramic content (50 wt%) and porosity (65-67 %). After 50 % compression, in an aqueous environment at 37 degrees C, tested samples returned to 95 % of their original height. Hydrolytic degradation of beta-TCP/PLCL composite, during the 24-week follow-up, was very similar to that of porous PLCL scaffold both in vitro and in vivo. Osteogenic differentiation of hASCs was demonstrated by alkaline phosphatase activity analysis, alizarin red staining, soluble collagen analysis, immunocytochemical staining and qRT-PCR. In vitro, hASCs formed a pronounced mineralised collagen matrix. A rabbit femur defect model confirmed biocompatibility of the composite. According to histological Masson-Goldner's trichrome staining and micro-computed tomography, beta-TCP/PLCL composite did not elicit infection, formation of fibrous capsule or cysts. Finally, native bone tissue at 4 weeks was already able to grow on and in the beta-TCP/PLCL composite. The elastic and highly porous beta-TCP/PLCL composite is a promising bone substitute because it is osteoconductive and easy-to-use and mould intraoperatively. |
| Databáze: | OpenAIRE |
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