Polyacrylamide gel electrophoresis of humic and fulvic acids after acid hydrolysis

Autor:	Trubetskaya, O. E., Trubetskoj, O. A., Sáiz-Jiménez, Cesáreo
Rok vydání:	2001
Předmět:	Molecular size distribution Polyacrylamide gel electrophoresis Fulvic acids Humic acids Acid hydrolysis complex mixtures
Zdroj:	Digital.CSIC. Repositorio Institucional del CSIC instname
Popis:	Two soil humic acids (HA), a marine sediment HA, and a soil fulvic acid (FA), were fractionated by polyacrylamide gel electrophoresis in the presence of denaturing agents before and after 6N HCl hydrolysis. After acid hydrolysis, the intensity of the high molecular size (MS) fraction decreased considerably in all HAs. On the other hand, a new high-MS fraction appeared in the FA after hydrolysis. The electrophoretic data indicate that acid hydrolysis produced a transformation in the humic macromolecule, inducing either depolymerization or condensation, depending on the nature of the humic fraction. Therefore, the advantages and disadvantages of using acid hydrolysis should be carefully considered before this treatment is carried out.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=dedup_wf_001::accfcdc975b15ed24ec98a154b0d76ef http://hdl.handle.net/10261/58676 Zobrazit plný text záznamu