Quantitative T-2 Mapping of the Mouse Heart by Segmented MLEV Phase-Cycled T-2 Preparation

Autor: Coolen, Bram F., Simonis, Frank F. J., Geelen, Tessa, Moonen, Rik P. M., Arslan, Fatih, Paulis, Leonie E. M., Nicolay, Klaas, Strijkers, Gustav J.
Přispěvatelé: Radiology and Nuclear Medicine, ACS - Amsterdam Cardiovascular Sciences, CCA -Cancer Center Amsterdam, Biomedical Engineering and Physics, MUMC+: DA BV Klinisch Fysicus (9), Beeldvorming, RS: CARIM - R3 - Vascular biology
Jazyk: angličtina
Rok vydání: 2014
Předmět:
Zdroj: Magnetic resonance in medicine, 72(2), 409-417. John Wiley and Sons Inc.
Magnetic Resonance in Medicine, 72(2), 409-417. Wiley
ISSN: 0740-3194
Popis: Purpose: A high-quality, reproducible, multi-slice T-2-mapping protocol for the mouse heart is presented. Methods: A T-2-prepared sequence with composite 90 degrees and 180 degrees radiofrequency pulses in a segmented MLEV phase cycling scheme was developed. The T-2-mapping protocol was optimized using simulations and evaluated with phantoms. Results: Repeatability for determination of myocardial T-2 values was assessed in vivo in n = 5 healthy mice on 2 different days. The average baseline T-2 of the left ventricular myocardium was 22.5 +/- 1.7 ms. The repeatability coefficient for R-2=1/T-2 for measurements at different days was Delta R-2=6.3 s(-1). Subsequently, T-2 mapping was applied in comparison to late-gadolinium-enhancement (LGE) imaging, to assess 1-dayold ischemia/reperfusion (IR) myocardial injury in n 8 mice. T-2 in the infarcts was significantly higher than in remote tissue, whereas remote tissue was not significantly different from baseline. Infarct sizes based on T-2 versus LGE showed strong correlation. To assess the time-course of T-2 changes in the infarcts, T-2 mapping was performed at day 1, 3, and 7 after IR injury in a separate group of mice (n 16). T-2 was highest at day 3, in agreement with the expected time course of edema formation and resolution after myocardial infarction. Conclusion: T-2 prepared imaging provides high quality reproducible T-2 maps of healthy and diseased mouse myocardium. (C) 2013 Wiley Periodicals, Inc
Databáze: OpenAIRE
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