Radiolabeling by aluminum fluoride-18 complexation: method optimization on a NODA-based model for application to sensitive biomolecule as PET tracers for oncology
| Autor: | San, Carine, Vignal, Nicolas, Hosten, Benoit, Port, Marc, Sarda-Mantel, Laure, Dioury, Fabienne |
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| Přispěvatelé: | Laboratoire Génomique, bioinformatique et chimie moléculaire (GBCM), Conservatoire National des Arts et Métiers [CNAM] (CNAM), Institut de Recherche Saint-Louis - Hématologie Immunologie Oncologie (Département de recherche de l’UFR de médecine, ex- Institut Universitaire Hématologie-IUH) (IRSL), Université de Paris (UP), Service de Neuroradiologie [CHU Lariboisière], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Lariboisière-Fernand-Widal [APHP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Hopital Saint-Louis [AP-HP] (AP-HP), Dioury, Fabienne |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: | |
| Zdroj: | Société de chimie Thérapeutique, 28th Young Research Fellows Meeting /Journées Jeunes Chercheurs Société de chimie Thérapeutique, 28th Young Research Fellows Meeting /Journées Jeunes Chercheurs, Feb 2021, Paris (en ligne), France |
| Popis: | International audience; Fluorine-18 (18F) is the most favorable positon emitter for tumor imaging. However, direct 18F labeling of biomolecules is challenging as it involves multiple steps and stringent conditions that are generally not suitable for biomolecules whose integrity may be altered. Over the past decade, an elegant new approach has been developed by coordination of aluminum fluoride {Al-18F}.(a) The objective of this work was to implement such a method and to find the mildest conditions compatible with heat- or acid-sensitive biomolecules. To evaluate {Al-18F} complexation, NODA MP C4 was prepared as a model compound from the commercially available NODA MP NCS (Figure). This model bears a thiourea function to mimic that present in the final conjugates. The corresponding radioactive complex Al18F NODA MP C4 was obtained following the most common reported radiofluorination conditions (pH 4, buffer AcO/AcOH, reflux). Based on this preliminary result, several parameters have been varied to obtain the highest radiochemical yield (RCY) under the mildest conditions. To this end and to guide the operational conditions in radiochemistry, a complementary study in cold series was performed in parallel. Al18F NODA MP C4 was obtained with a reproducible satisfactory RCY on comparison with reported results on NODA derivatives.(b,c) Herein, we will present and discuss the optimization study. |
| Databáze: | OpenAIRE |
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