The biological response to nanometre-sized polymer particles
| Autor: | Liu, Aiqin, Richards, Laura, Bladen, Catherine L., Ingham, Eileen, Fisher, John, Tipper, Joanne L. |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Adult
Male Dose-Response Relationship Drug Joint replacement Biomedical Engineering Osteolysis Middle Aged Bioactivity Biochemistry Biomaterials Young Adult Nanoparticle Polyethylene Materials Testing Leukocytes Mononuclear Humans Immunologic Factors Cytokines Nanoparticles Female Polyethylenes Particle Size Molecular Biology Cells Cultured Biotechnology |
| ISSN: | 1742-7061 |
| Popis: | Recently, nanometre-sized UHMWPE particles generated from hip and knee replacements have been identified in vitro and in vivo. UHMWPE particles in the 0.1-1.0 µm size range have been shown to be more biologically active than larger particles, provoking an inflammatory response implicated in late aseptic loosening of total joint replacements. The biological activity of nanometre-sized particles has not previously been studied. The biological response to clinically-relevant UHMWPE wear particles including nanometre-sized and micrometre-sized, along with polystyrene particles (FluoSpheres 20 nm, 60 nm, 200 nm and 1.0 µm), and nanometre-sized model polyethylene particles (Ceridust 3615®), was determined in terms of osteolytic cytokine release from primary human peripheral blood mononuclear cells (PBMNC’s). Nanometre-sized UHMWPE wear particles, nanometre-sized Ceridust 3615® and 20 nm FluoSpheres had no significant effect on TNF-α, IL-1β, IL-6 and IL-8 release from PBMNC’s at a concentration of 100 μm3 particles per cell after 12 and 24 hours. The micrometre-size UHMWPE wear particles (0.1-1.0 μm) and 60 nm, 200 nm and 1.0 µm FluoSpheres caused significantly elevated osteolytic cytokine release from PBMNC’s. These results indicated that particles below circa 50 nm fail to activate PBMNC’s and that particle size, composition and morphology played a crucial role in cytokine release by particle stimulated macrophages. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect