Pregnancy in goats induces a strong transcriptomic change in the olfactory bulb

Autor: Luigi-Sierra, Maria Gracia, Guan, Dailu, López-Béjar, Manel, Casas, Eduard, Olvera, S., Gardela, J., Palomo, M. J., Osuagwuh, U. I., Ohaneje, U. L., Mármol-Sánchez, Emilio, Amills, Marcel
Jazyk: angličtina
Rok vydání: 2021
Zdroj: Digital.CSIC. Repositorio Institucional del CSIC
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Popis: Resumen del póster presentado a la 38th International Conference on Animal Genetics (ISAG), celebrada virtualmente del 26 al 30 de julio de 2021.
The brain is an organ involved in essential processes such as the maintenance of body homeostasis, memory, learning, and behavior. Pregnancy induces physiological changes to allow the development of the embryo as well as the preparation of the female for motherhood, and the majority of these processes are tightly regulated in a brain-dependent manner. For instance, the establishment of offspring bonding and nurturing after parturition are regulated by behavioral changes at the central nervous system, which experiences substantial anatomical and transcriptomic changes. Studies performed in humans and mice have demonstrated that the brain of pregnant females experiences substantial anatomical and transcriptomic changes. In this work, we aimed to understand the transcriptomic changes induced by pregnancy in goats by bulk sequencing of total RNA from 12 brain regions in 3 pregnant (second month of gestation) and 4 nonpregnant Murciano-Granadina females. Subsequently, we extracted total mRNA from each tissue and performed sequencing. Quality control and removal of the adapters were carried out with FastQC and TrimGalore. Reads were aligned to the goat reference genome ARS1 with HISAT2 software, and mRNA expression profiles were estimated with StringTie tool. Differential expression analyses were performed with DESeq2. We observed that pregnancy does not significantly affect the transcriptomic profiles of the medulla oblongata, cerebellar trunk, cerebellar hemisphere, rostral collicle, hypothalamus, and neurohypophysis. In contrast, the olfactory bulb, adenohypophysis, pons, frontal neocortex, hippocampus, and pineal gland displayed significant changes in mRNA expression, with a total of 1,234, 203, 197, 85, 71, and 66 differentially expressed genes (DEGs), respectively. Among the 12 brain regions under analysis, the olfactory bulb transcriptome was the most heavily affected by pregnancy. These findings agree well with studies performed in mice during the 70s that demonstrated that bulbectomized mice during pregnancy neglected their litters and were unable to build well-constructed nests.
Databáze: OpenAIRE
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