Ca2+ signals during early lymphocyte activation in carp Cyprinus carpio L
| Autor: | Lidy van Kemenade, Saeij, J. P. J., Flik, G., Willems, P. H. G. M. |
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| Jazyk: | angličtina |
| Rok vydání: | 1998 |
| Předmět: |
Phytohaemagglutinin
Neurophysiology Celbiologie en Immunologie Lipopolysaccharide Mechanisme en functie van oscillerende second messenger systemen B-cell cross-linking Mechanism and function of oscillating second messenger systems Fluo3 Fish Cell Biology and Immunology Cyprinus carpio Digital imaging microscopy Carp WIAS Intracellular Ca2+ Organismal Animal Physiology Flow cytometry Lymphocytes Fura-2 GeneralLiterature_REFERENCE(e.g. dictionaries encyclopedias glossaries) |
| Zdroj: | Journal of Experimental Biology 201 (1998) Journal of Experimental Biology, 201, pp. 591-598 Journal of Experimental Biology, 201, 591-598 Scopus-Elsevier |
| ISSN: | 0022-0949 |
| Popis: | To measure cellular responses and the involvement of increased cytosolic Ca2 levels ([Ca2 ]i), peripheral blood leukocytes (PBL) of carp were loaded with the fluorescent intracellular Ca2 indicators Fluo-3 and Fura-2. Responses of lymphocytes to T-cell mitogen (phytohaemagglutinin, PHA), to B-cell mitogen (lipopolysaccharide, LPS) and to immunoglobulin (Ig) cross-linking with a monoclonal antibody to carp Ig were measured using flow cytometry. Both T-cell stimulation by PHA and B-cell stimulation by membrane Ig cross-linking evoked a rapid elevation of [Ca2 ]i. B-cell stimulation by LPS was not linked to an increase in [Ca2 ]i. As judged by the percentage of reacting cells, it was concluded that all Ig-positive lymphocytes reacted to Ig cross-linking by elevating [Ca2 ]i. At the single-cell level, the reactions of Fura-2-loaded cells were followed every 6 s using digital imaging microscopy. Both cells displaying spontaneous [Ca2 ]i oscillations and non-oscillating cells responded to stimulation with an increase in [Ca2 ]i, sometimes, in already oscillating cells, accompanied by an increase in frequency and/or amplitude of the oscillations. These results show that intracellular Ca2 responses of PBL upon activation resemble those in mammals and form a powerful tool for studies into cell-specific regulation. |
| Databáze: | OpenAIRE |
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