Combining Atomic Force Microscopy and genetics to investigate the role of Knr4 in Saccharomyces cerevisiae sensitivity to K9 Killer toxin

Autor: Yken, Helene, Liu, Ran, Formosa-Dague, Cécile, Schiavone, Marion, DAGUE, Etienne, François, Jean-Marie, Timmers Dagkessamanskaya, Adilya
Přispěvatelé: Laboratoire d'Ingénierie des Systèmes Biologiques et des Procédés (LISBP), Centre National de la Recherche Scientifique (CNRS)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut National de la Recherche Agronomique (INRA), Équipe NanoBioSystèmes (LAAS-NBS), Laboratoire d'analyse et d'architecture des systèmes (LAAS), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Université Toulouse 1 Capitole (UT1)-Université Toulouse - Jean Jaurès (UT2J)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Université Toulouse 1 Capitole (UT1)-Université Toulouse - Jean Jaurès (UT2J), Institut National de la Recherche Agronomique (INRA)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS), Université Toulouse - Jean Jaurès (UT2J)-Université Toulouse 1 Capitole (UT1), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Toulouse - Jean Jaurès (UT2J)-Université Toulouse 1 Capitole (UT1), Université Fédérale Toulouse Midi-Pyrénées
Jazyk: angličtina
Rok vydání: 2015
Předmět:
Zdroj: Letters in Applied NanoBioScience
Letters in Applied NanoBioScience, 2015, 4 (4), pp.306-315
ISSN: 2284-6808
Popis: International audience; K9 killer toxin is a small peptide secreted by the yeaststrong cytocidal activity against sensitive yeast strains, includingformation of pores at the surface of the cells, and more specifically at places where cell wall synthesis is the most active,tip of growing buds or mating projections. Yeast cells treated withese pores. In the yeast S. cerevisiae, KNR4 protein localizes at the sites of polarized growth (bud tips, shmoo tips), which are also thesites where the toxin forms pores in the cell wall. Mutants defective inanalyzed for the first time the biophysical effects of K9 on the yeast cell wall using Atomic Force Microscopy (AFM),technology that allows measuring the nanomechanicalwe measured the effects of K9 toxin on the nanomechanical properties of the cell wall ofdeleted for KNR4 gene, at the short (2 h) and long term (20 h).type cells already after 2 hours and only visible inprotein in the cells sensitivity towards the toxin.which is required for its correct cellular localization at the bud tip during cell cycle, is essential for the toxin Kaddition, a series of deletion mutants from the YKO collection in which the Knr4 cellular localization is also lost display asensitivity to the K9 toxin. Taken together, these results shed light on the importance of theintensive cell wall growth for the wild-type cells sensitivity to K9 killer toxin.
Databáze: OpenAIRE
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