Characterisation of an acetylcholine receptor gene of Haemonchus contortus in relation to levamisole resistance

Autor: Hoekstra, R., Visser, A., Wiley, Lj, ANTHONY WEISS, Sangster, Nc, Roos, Mh
Přispěvatelé: Other departments
Jazyk: angličtina
Rok vydání: 1997
Předmět:
Zdroj: Molecular and biochemical parasitology 84 (1997) 2
Molecular and biochemical parasitology, 84(2), 179-187. Elsevier
Molecular and biochemical parasitology, 84(2), 179-187
ResearcherID
ISSN: 0166-6851
Popis: The anthelmintic drug levamisole is thought to bind to nicotinic acetylcholine receptors of nematodes. It is possible that resistance to this drug is associated with either a change in binding characteristics or a reduction in the number of nicotinic acetylcholine receptors. Therefore, the molecular mechanism of levamisole resistance in the parasitic nematode Haemonchus contortus was studied by isolating and characterising cDNA clones encoding a putative ligand binding nicotinic acetylcholine receptor subunit, HCA1, of two susceptible and one levamisole resistant population. HcA1 is related to unc-38, a nicotinic acetylcholine receptor subunit gene associated with levamisole resistance in Caenorhabditis elegans. Although extensive sequence analyses of hca1 sequences revealed poly-morphism at amino acid level, no association with levamisole resistance could be detected. Restriction fragment length polymorphism analyses confirmed that, although polymorphism was detected, no selection of a specific allele of heal has taken place during selection for levamisole resistance in various levamisole resistant populations.
Databáze: OpenAIRE
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