Chemical composition and bioactivity potential of the new endosequence BC Sealer formulación hiflow

Autor: Rodríguez Lozano, Francisco Javier, López-García, Sergio, García-Bernal, David, Tomás-Catalá, C.J., Santos, J.M., Llena Puy, María Carmen, Lozano, A., Murcia, L., Forner Navarro, Leopoldo
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Zdroj: Rodríguez Lozano, Francisco Javier López-García, Sergio García-Bernal, David Tomás-Catalá, C.J. Santos, J.M. Llena Puy, María Carmen Lozano, A. Murcia, L. Forner Navarro, Leopoldo 2020 Chemical composition and bioactivity potential of the new endosequence BC Sealer formulación hiflow International Endodontic Journal 53 9 1216 1228
RODERIC. Repositorio Institucional de la Universitat de Valéncia
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Popis: Aim To evaluate in a laboratory setting the effects of Endosequence BC Sealer HiFlow (Brasseler USA, Savannah, GA, USA), a novel calcium silicate-based sealer developed for use in warm canal filling techniques, on human periodontal ligament stem cells (hPDLSCs). Methodology Eluates of EndoSequence BC Sealer HiFlow (BCHiF) (Brasseler USA), EndoSequence BC Sealer (BCS) (Brasseler USA) and AH Plus (AHP) (Dentsply DeTrey GmbH, Konstanz, Germany), were placed in contact with hPDLSCs. The characterisation of the chemical elements of the root canal sealers was assessed using Scanning Electron Microscopy and Energy Dispersive X-ray analysis (SEM-EDX). Inductively Coupled Plasma Mass Spectrometry (ICP-MS) was used to determine the ion release of the sealers. MTT assay and wound healing techniques were used to determine cell viability and migration, respectively. Cell morphology and cell attachment were assessed using a direct contact technique of hPDLSCs onto the surface of the sealers and analysed by SEM. The bioactivity potential was carried out with the Alizarin Red and qPCR testing methods. The statistical differences were evaluated using one-way ANOVA and Tukey´s test (p0.05). Both BCS and BCHiF had similar rates of cell migration to the control group at 24 and 48 hours. Cell morphology and adhesion capacity were also similar for BCS and BCHiF groups, while the AHP group was associated with reduced adhesion capacity. The Alizarin Red assay revealed a significant difference between the BCS and the control group (p
Databáze: OpenAIRE