Presence of Small Ruminant Lentiviruses in aluminium-induced, post-vaccination granulomas in sheep

Autor: Miguel, Ricardo de, Echeverría, Irache, Asín, Javier, Molín, Jéssica, Pablo, Lorena de, Fernández, Antonio, Lacasta, Delia, Andrés, Damián F. de, Pérez, Marta M., Luján, Lluís, Reina, Ramsés
Přispěvatelé: Ministerio de Economía y Competitividad (España), Gobierno de Aragón
Rok vydání: 2019
Zdroj: Digital.CSIC. Repositorio Institucional del CSIC
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Popis: Resumen del trabajo presentado en el XXX Keele Meeting on Aluminium, celebrado en Yucatán (México), del 23 al 27 de marzo de 2019
In sheep, aluminium-containing vaccines induce the recruitment of macrophages and formation of granulomas1. Small ruminant lentiviruses (SRLV) are retroviruses that replicate in activated macrophages2. Eleven adult, naturally SRLV-infected sheep were inoculated with eight doses of aluminium-containing vaccines. In vitro cultures of granuloma, spleen, peripheral blood and bronchoalveolar lavage macrophages were established and monitored for retrotranscriptase activity, PCR and virus identification by gag gene sequencing. Immunohistochemistry was also performed with CAEP5A1 monoclonal antibody. SRLV could be detected in post-vaccination granulomas in 5 sheep (45.5%). In three animals, viral sequences in the granuloma were highly divergent compared to sequences isolated elsewhere in the same sheep (compartmentalization). Location of SRLV was mostly associated to Al-containing phagolysosomes. Persistent granulomatous inflammatory reactions induced by vaccines may allow in situ SRLV replication, thus favouring mutation and recombination. The role of Al-induced, post-vaccination granulomas in the spread and pathogenesis of SRLV need to be addressed and considered.
This work was funded by grants from the Spanish Ministry of Economy and Industry (AGL2013-49137-C3-1-R and AGL2013-49137-C3-2-R). De Miguel R is a PhD student funded by the Department of Innovation, Research and University of Aragon.
Databáze: OpenAIRE
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