The effect of changing temperature and agar concentration at proliferation stage in the final success of Aleppo pine somatic embryogenesis

Autor: Pereira, Catia, Montalbán, Itziar A., Goicoa Mangado, Tomás, Ugarte Martínez, María Dolores, Correia, Sandra, Canhoto, Jorge M., Moncaleán, Paloma
Přispěvatelé: Universidad Pública de Navarra. Departamento de Estadística e Investigación Operativa, Nafarroako Unibertsitate Publikoa. Estatistika eta Ikerketa Operatiboa Saila
Rok vydání: 2017
Předmět:
Zdroj: Academica-e. Repositorio Institucional de la Universidad Pública de Navarra
instname
Academica-e: Repositorio Institucional de la Universidad Pública de Navarra
Universidad Pública de Navarra
Popis: Aim of the study: The effect of physical and chemical conditions at proliferation stage was evaluated in order to elucidate if this stage is the determinant phase to induce a marked effect in Pinus halepensis somatic embryogenesis. Area of study: The study was conducted in research laboratories of Neiker (Arkaute, Spain). Material and methods: Pinus halepensis embryonal masses from ten embryogenic cell lines subjected to nine treatments (tissues cultured at three temperatures on media supplemented with three agar concentrations) at proliferation stage. Main results: Significant differences were observed among different proliferation conditions months later at the end of maturation, germination and acclimatization stages. Research highlights: Aleppo pine embryonal masses are cultured under standard conditions on a culture medium supplemented with 4.5 g/L Gelrite® at 23ºC. However, better results in terms of plantlet production can be obtained proliferating the embryonal masses at 18ºC in a culture media with significantly lower water availability. MINECO, Spanish Government (project AGL2013-4700-C4-2R).
Databáze: OpenAIRE