Subpallial Enhancer Transgenic Lines: a Data and Tool Resource to Study Transcriptional Regulation of GABAergic Cell Fate
Autor: | Silberberg, SN, Taher, L, Lindtner, S, Sandberg, M, Nord, AS, Vogt, D, Mckinsey, GL, Hoch, R, Pattabiraman, K, Zhang, D, Ferran, JL, Rajkovic, A, Golonzhka, O, Kim, C, Zeng, H, Puelles, L, Visel, A, Rubenstein, JLR |
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Rok vydání: | 2016 |
Předmět: |
Homeodomain Proteins
Neurology & Neurosurgery Enhancer Elements 1.1 Normal biological development and functioning LIM-Homeodomain Proteins Neurosciences Stem Cell Research - Embryonic - Non-Human Nerve Tissue Proteins Cell Differentiation Stem Cell Research Basal Ganglia Transgenic Mice Gene Expression Regulation Genetic Underpinning research Genetics Animals Psychology Developmental Cognitive Sciences GABAergic Neurons Transcription Factors Biotechnology |
Zdroj: | Neuron, vol 92, iss 1 Silberberg, SN; Taher, L; Lindtner, S; Sandberg, M; Nord, AS; Vogt, D; et al.(2016). Subpallial Enhancer Transgenic Lines: a Data and Tool Resource to Study Transcriptional Regulation of GABAergic Cell Fate. Neuron, 92(1), 59-74. doi: 10.1016/j.neuron.2016.09.027. UCSF: Retrieved from: http://www.escholarship.org/uc/item/79k1j6xd |
DOI: | 10.1016/j.neuron.2016.09.027. |
Popis: | © 2016 Elsevier Inc. Elucidating the transcriptional circuitry controlling forebrain development requires an understanding of enhancer activity and regulation. We generated stable transgenic mouse lines that express CreERT2and GFP from ten different enhancer elements with activity in distinct domains within the embryonic basal ganglia. We used these unique tools to generate a comprehensive regional fate map of the mouse subpallium, including sources for specific subtypes of amygdala neurons. We then focused on deciphering transcriptional mechanisms that control enhancer activity. Using machine-learning computations, in vivo chromosomal occupancy of 13 transcription factors that regulate subpallial patterning and differentiation and analysis of enhancer activity in Dlx1/2 and Lhx6 mutants, we elucidated novel molecular mechanisms that regulate region-specific enhancer activity in the developing brain. Thus, these subpallial enhancer transgenic lines are data and tool resources to study transcriptional regulation of GABAergic cell fate. |
Databáze: | OpenAIRE |
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