A gene‐for‐gene interaction involving a ‘late’ effector contributes to quantitative resistance to the stem canker disease in Brassica napus

Autor: JIQUEL, Audren, Gervais, Julie, Geistodt‐Kiener, Aude, Delourme, Régine, GAY, Elise, Ollivier, Bénédicte, Fudal, Isabelle, Faure, Sébastien, Balesdent, Marie‐Hélène, Rouxel, Thierry
Přispěvatelé: BIOlogie et GEstion des Risques en agriculture (BIOGER), AgroParisTech-Université Paris-Saclay-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Euralis Semences, Université Paris Saclay, Institut de Génétique, Environnement et Protection des Plantes (IGEPP), Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), innolea, 2017/1374, Association Nationale de la Recherche et de la Technologie, SOFIPROTEOL, Terres Inovia, INRAE SPE, ANR-17-EURE-0007,SPS-GSR,Ecole Universitaire de Recherche de Sciences des Plantes de Paris-Saclay(2017), Université de Rennes (UR)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-INSTITUT AGRO Agrocampus Ouest, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Zdroj: New Phytologist
New Phytologist, Wiley, 2021, ⟨10.1111/nph.17292⟩
New Phytologist, 2021, 231 (4), pp.1510-1524. ⟨10.1111/nph.17292⟩
ISSN: 0028-646X
1469-8137
DOI: 10.1111/nph.17292⟩
Popis: Early Access; International audience; The control of stem canker disease of Brassica napus (rapeseed), caused by the fungus Leptosphaeria maculans is largely based on plant genetic resistance: single-gene specific resistance (Rlm genes) or quantitative, polygenic, adult-stage resistance. Our working hypothesis was that quantitative resistance partly obeys the gene-for-gene model, with resistance genes "recognizing" fungal effectors expressed during late systemic colonization. Five LmSTEE (stem-expressed effector) genes were selected and placed under the control of the AvrLm4-7 promoter, an effector gene highly expressed at the cotyledon stage of infection, for miniaturized cotyledon inoculation test screening of a gene pool of 204 rapeseed genotypes. We identified a rapeseed genotype, 'Yudal', expressing hypersensitive response to LmSTEE98. The LmSTEE98-RlmSTEE98 interaction was further validated by inactivation of the LmSTEE98 gene with a CRISPR-Cas9 approach. Isolates with mutated versions of LmSTEE98 induced more severe stem symptoms than the wild-type isolate in 'Yudal'. This single-gene resistance was mapped in a 0.6 cM interval of the 'Darmor_bzh' x 'Yudal' genetic map. One typical gene-for-gene interaction contributes partly to quantitative resistance when L. maculans colonizes the stems of rapeseed. With numerous other effectors specific to stem colonization, our study provides a new route for resistance gene discovery, elucidation of quantitative resistance mechanisms, and selection for durable resistance.This article is protected by copyright. All rights reserved.
Databáze: OpenAIRE
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